2012 Fiscal Year Final Research Report

Analysis of regulation mechanism for metabolic pathway based on comparative proteomics for protein abundance

Research Project

Project/Area Number	22510207
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Genome biology
Research Institution	Meiji University
Principal Investigator	KITO Keiji 明治大学, 農学部, 専任講師 (40345632)
Project Period (FY)	2010 – 2012
Keywords	比較プロテオミクス / 代謝経路 / 酵母 / 質量分析
Research Abstract	In this study, we analyzed proteome expression pattern for multiple yeast species grown on different culture conditions to understand proteome differences involved in interspecies varieties. Eight yeast species grew with comparable growth rate on glucose medium. In contrast, four species of Saccharomyces genus, including S. cerevisiae, showed slow growth rate by two-to three-fold on glycerol and acetate medium, compared to four species belonging to different genus. Some groups of proteins involved in mitochondrial metabolic pathway and response to oxidative stress showed different abundance between Saccharomyces and other genus when grown on glycerol. It is suggested that such differences in abundance of proteins with specific functions could be responsible for disparity of growth rate among yeast species.