ERas regulated apoptosis in ES cells through Rho pathway.

2013 Fiscal Year Final Research Report

• Project/Area Number: 22510212
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical genome science
• Research Institution: Akita University (2011-2013); Jichi Medical University (2010)
• Principal Investigator: IKEDA TAMAKO 秋田大学, 学内共同利用施設等, 助教 (10406035)
• Co-Investigator(Renkei-kenkyūsha): HANAZONO Yutaka 自治医科大学, 医学部, 教授 (70251246)
• Project Period (FY): 2010-04-01 – 2013-03-31
• Keywords: ERas遺伝子 / Rho/ROCK経路 / アポトーシス / ROCK阻害薬 / ES細胞

Research Abstract

ERas promotes proliferation of mouse embryonic stem cells, however human ES cells hardly express the ERas gene. Human ES cells , poor survival after cell dissociation is a major obstacle to research, hindering manipulation such as sorting and cloning of cells. On the other hand, treatment of hES cells with ROCK inhibitor markedly diminished apoptosis, enabling hES cells to propagate as mouse ES cells do following dissociation(Watanabe et al.,2007). We examined the relationship between ERas and the Rho/ROCK pathway. Results, our study have revealed a previously unknown pathway that inactivated the Rho/ROCK pathway in ES cells to suppress apotheosis.

Research Products

• [Journal Article] Ex vivo expansion of human HSCs with Sendai virus vector expressing HoxB4 assessed by sheep in utero transplantation (2011)
  • Author(s): Abe T, Masuda S, Ban H, Hayashi S, Ueda Y, Inoue M, Hasegawa M, Nagao Y, Hanazono Y
  • Journal Title: Exp Hematol Volume: 39(1) Pages: 47-54
• [Journal Article] Migration of cells from the yolk sac to hematopoietic tissues after in utero transplantation of early and mid gestation canine fetuses (2011)
  • Author(s): Masuda S, Hayashi S, Ageyama N, Shibata H, Abe T, Nagao Y, Hanazono Y
  • Journal Title: Transplantation Volume: 92(2) Pages: e5-6
• [Journal Article] Intravascular route is not superior to an intraperitoneal route for in utero transplantation of human hematopoietic stem cells and engraftment in sheep (2010)
  • Author(s): Tanaka Y, Masuda S, Abe T, Hayashi S, Kitano Y, Nagao Y, Hanazono Y
  • Journal Title: Transplantation Volume: 90(4) Pages: 462-3
  • Peer Reviewed
• [Presentation] 実験動物に対する消化管運動バルーン計測法の検討 (2013)
  • Author(s): 長縄明大,小松和、池田たま子,松田幸久、岡潔
  • Organizer: 第15回日本消化器病学会
  • Place of Presentation: 出雲
  • Year and Date: 20131100
• [Presentation] 緑膿菌等の細菌を用いた微酸性水の殺菌・消毒効果の検証 (2013)
  • Author(s): 小畑孝弘,柴田淑子,川越政美,二部恒美,池田勝久,佐藤政義,戸井田和実,助川康子,池田たま子,松田幸久
  • Organizer: 実験動物技術者協会第47回総会in晴れの国岡山
  • Place of Presentation: 岡山市
  • Year and Date: 20130900
• [Presentation] 再生医学:iPS細胞と実験動物における最近の知見 (2013)
  • Author(s): 池田たま子
  • Organizer: 実験動物技術者協会奥羽支部勉強会
  • Place of Presentation: 秋田市(特別講演)
  • Year and Date: 20130400
• [Presentation] セリウスソフト水の長期摂取がB6マウスの繁殖等に及ぼす影響について (2012)
  • Author(s): 川越政美,小畑孝弘,戸井田和実,九島秀美,柴田淑子,池田勝久,佐藤政義,二部恒美,池田たま子,松田幸久
  • Organizer: 日本実験動物技術者協会平成24年度奥羽・東北支部合同勉強会
  • Place of Presentation: 盛岡
  • Year and Date: 20121100
• [Presentation] 秋田大学動物実験部門での蟯虫感染についての事例 (2012)
  • Author(s): 助川康子,二部恒美,川越政美,柴田淑子,池田勝久,佐藤政義,小畑孝弘,戸井田和実,鈴木美帆子,九島秀美,津谷優子,池田たま子,松田幸久
  • Organizer: 日本実験動物技術者協会平成24年度奥羽・東北支部合同勉強会
  • Place of Presentation: 盛岡市
  • Year and Date: 20121100
• [Presentation] 秋田大学におけるSPF動物の飼育状況と検疫・微生物モニタリングの現状 (2012)
  • Author(s): 池田たま子,松田幸久
  • Organizer: 第38回国立大学実験動物施設協議会全国大会サテライトシンポジウム
  • Place of Presentation: 秋田市
  • Year and Date: 20120500
• [Presentation] 秋田大学におけるSPFマウス飼育エリア(A)の飼育環境改善の取組みと工夫 (2011)
  • Author(s): 戸井田和実、池田たま子、二部恒美、鈴木美帆子、佐藤正義、小畑孝弘、九島秀美、川越政美、助川康子、池田勝久、柴田淑子、津谷優子、松田幸久
  • Organizer: 平成23年日本実験動物技術者協会奥羽・東北支部合同勉強会
  • Year and Date: 20111100