2012 Fiscal Year Final Research Report
System level analysis of epigenomic regulation in mammalian embryo
| Project/Area Number |
22510220
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
System genome science
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| Research Institution | Kyoto University (2012)
The Institute of Physical and Chemical Research (2010-2011) |
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Principal Investigator |
KUMAKI Yuichi 京都大学, 医学研究科, 助教 (50455341)
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| Project Period (FY) |
2010 – 2012
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| Keywords | エピゲノム制御 / エピジェネティクス / DNA メチル化 / ヒストン修飾 / システムバイオロジー / バイオインフォマティクス |
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| Research Abstract |
Cytosine methylation at CpG site of genomic DNA is indispensable for mammalian development. However, a direct role of DNA methylation remains unclear. To reveal the effect of DNA methylation, we performed genome-wide analysis of gene expression and histone modifications of ES cells, differentiated me sodermal cells, nuclear transferred TS cells deficient for DNA methyltransferases. We found that the effect of DNA methylation loss on global expression profiles are modest, while global tendency of promoter histone modifications, especially H3K27me3, is sensitive to DNA methylation.
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