2012 Fiscal Year Final Research Report
PRODUCTION OF NEW NATURAL PRODUCTS BY BIOSYNTHETIC ENGINEERING
| Project/Area Number |
22510225
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Nigata University of Phermacy and Applied Life Sciences |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Keywords | 生合成 / 人為的制御 / トリテルペン合成酵素 / ジオキシドスクアレン / アキレオール / マーネラル |
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| Research Abstract |
Skeleton of triterpene is formed from oxidosqualene by triterpene synthase. Many triterpene synthases give tetra- or penta-cyclic products, while marneral synthase and achilleol synthase give a mono-cyclic product. In this study, these two enzymes were expressed in the yeast where di-oxidosqualene was accumulated to obtain a new natural triterpene with two separate rings which was produced by cyclization of di-oxidosqualene from both ends. TLC analysis showed that the target compound was produced by the transformed yeast with squalene epoxidase and achilleol synthase genes.
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