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2012 Fiscal Year Final Research Report

Function of antifreeze protein and dynamic crossover of hydration water

Research Project

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Project/Area Number 22550024
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical chemistry
Research InstitutionFukuoka University

Principal Investigator

YOSHIDA Koji  福岡大学, 理学部, 助教 (00309890)

Co-Investigator(Renkei-kenkyūsha) YAMAGUCHI Toshio  福岡大学, 理学部, 教授 (70158111)
Project Period (FY) 2010 – 2012
Keywords生物物理 / 中性子散乱 / タンパク質 / 水和
Research Abstract

The correlation between the antifreeze effect of AFP and the structure and dynamics of hydration water of the protein was studied by X-ray and neutron scatterings (quasi-elastic neutron scattering, inelastic X-ray scattering, and X-ray diffraction) and MD simulation. Although the dynamic transition of the hydrated protein was observed, water structure of hydration water changed insignificantly. Compared with the result of hydration water of the other proteins and confined water in mesoporous materials, the transition between high density water and low density water was discussed.

  • Research Products

    (18 results)

All 2013 2012 2011 2010

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (8 results)

  • [Journal Article] Thermal behavior, structure, and dynamics of low-temperature water confined in mesoporous organosilica by differential scanning calorimetry, X-ray diffraction, and quasi-elastic neutron scattering2013

    • Author(s)
      M. Aso, K. Ito, H. Sugino, K. Yoshida, T. Yamada, O. Yamamuro, S. Inagaki, T. Yamaguchi
    • Journal Title

      Pure Appl. Chem

      Volume: 85 Pages: 289-305

    • DOI

      DOI:10.1351/PAC-CON-12-06-02

    • Peer Reviewed
  • [Journal Article] Thermal behavior and structure of low-temperature water confined in Sephadex G15 Gel by differential scanning calorimetry and large-angle X-ray diffraction measurements2013

    • Author(s)
      K. Ito, K. Yoshida, K. Ujimoto, T. Yamaguchi
    • Journal Title

      Anal. Sci

      Volume: 29 Pages: 353-359

    • DOI

      DOI:10.2116/analsci.29.353

    • Peer Reviewed
  • [Journal Article] Alcohol Induced Structural and Dynamic Changes in β-Lactoglobulin in Aqueous Solution: A Neutron Scattering Study2012

    • Author(s)
      K. Yoshida, K. Vogtt, Z. Izaola, M. Russina, T. Yamaguchi, M.-C. Bellissent-Funel
    • Journal Title

      BBA - Proteins and Proteomics

      Volume: 1824 Pages: 502-510

    • DOI

      DOI:10.1016/j.bbapap.2011.12.011

    • Peer Reviewed
  • [Journal Article] Structure of Hexafluoroisopropanol Water Mixtures by Molecular Dynamics Simulations2012

    • Author(s)
      T. Yamaguchi, S. Imura T. Kai, K. Yoshida
    • Journal Title

      Z. Naturforsch.

      Volume: 68a Pages: 145-151

    • DOI

      DOI:10.5560/ZNA.2012-0100

    • Peer Reviewed
  • [Journal Article] メソ ポーラスシリカならびに規則性メソポーラ ス有機シリカ内に閉じ込められた水の構造 とダイナミクス2012

    • Author(s)
      吉田亨次・早田葵・麻生真以・伊藤華 苗・橘高茂治・稲垣伸二・山口敏男
    • Journal Title

      分析化学

      Volume: 61(12) Pages: 989-998

    • DOI

      DOI:10.2116/bunsekikagaku.61.989

    • Peer Reviewed
  • [Journal Article] Neutron spic echo measurements of monolayer and capillary condensed water in MCM-41 at low temperatures2012

    • Author(s)
      K. Yoshida, T. Yamaguchi, S. Kittaka, M.-C. Bellissent-Funel, P. Fouquet
    • Journal Title

      J. Phys: Condens. Matter

      Volume: 24 Pages: 064101

    • DOI

      DOI:10.1088/0953-8984/24/6/064101

    • Peer Reviewed
  • [Journal Article] X-ray diffraction study on monolayer and capillary-condensed acetonitrile in mesoporous MCM-41 at low temperatures2011

    • Author(s)
      T. Yamaguchi, H. Sugino, K. Ito, K. Yoshida, S. Kittaka
    • Journal Title

      J. Mol. Liq.

      Volume: 164 Pages: 53-58

    • DOI

      DOI:10.4153/CMB-2011-148-0

    • Peer Reviewed
  • [Journal Article] メソポーラス物質MCM-41中に閉じ込められた低温水の熱挙動,構造とダイナミクス2011

    • Author(s)
      山口敏男,吉田亨次,伊藤華苗,橘高茂治,高原周一
    • Journal Title

      分析化学

      Volume: 60 Pages: 115-130

    • DOI

      DOI:10.2116/bunsekikagaku.60.115

    • Peer Reviewed
  • [Journal Article] A Study of alcohol-induced gelation of beta-lactoglobulin with small-angle neutron scattering, neutron spin echo, and dynamic light scattering measurements2010

    • Author(s)
      K. Yoshida, T. Yamaguchi, N. Osaka, H. Endo, M. Shibayama
    • Journal Title

      Phys. Chem. Chem. Phys.

      Volume: 12 Pages: 3260-3269

    • DOI

      DOI:10.1039/b920187d

    • Peer Reviewed
  • [Journal Article] Collective dynamics of hydrated β-lactoglobulin by inelasticx-ray scattering2010

    • Author(s)
      K. Yoshida, S. Hosokawa, A.Q.R. Baron, T. Yamaguchi
    • Journal Title

      J. Chem. Phys

      Volume: 133(13) Pages: 134501

    • DOI

      DOI:10.1063/1.3484238

    • Peer Reviewed
  • [Presentation] 低温におけるβ-ラクトグロブリンの水和水の構造2013

    • Author(s)
      吉田亨次,吉田絵里,伊藤華苗,山口敏男
    • Organizer
      日本化学会第93春季年会
    • Place of Presentation
      草津
    • Year and Date
      2013-03-23
  • [Presentation] X線非弾性散乱による水和タンパク質の集団ダイナミクス2012

    • Author(s)
      吉田亨次,山口敏男,A.Q.R.Baron
    • Organizer
      第35回溶液化学シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2012-11-13
  • [Presentation] β-ラクトグロブリンの会合状態に対するアルコールの影響2012

    • Author(s)
      吉田亨次、山口敏男
    • Organizer
      分子科学討論会2012
    • Place of Presentation
      東京
    • Year and Date
      2012-09-20
  • [Presentation] Alcohol effect on structure, dynamics, and aggregation of peptide and protein2012

    • Author(s)
      K. Yoshida, T. Yamaguchi
    • Organizer
      EMLG/JMLG annual meeting
    • Place of Presentation
      Eger (Hungary)
    • Year and Date
      2012-09-07
  • [Presentation] メソ多孔体中の水の状態分析2012

    • Author(s)
      吉田亨次
    • Organizer
      第72回分析化学討論会「分析化学における現代の溶液反応化学」
    • Place of Presentation
      鹿児島
    • Year and Date
      2012-05-20
  • [Presentation] 水和タンパク質のX線非弾性散乱2012

    • Author(s)
      吉田亨次,山口敏男
    • Organizer
      第25回日本放射光学会年会
    • Place of Presentation
      佐賀
    • Year and Date
      2012-01-07
  • [Presentation] Structure and dynamics of low-temperature water confined in porous silica2011

    • Author(s)
      K. Yoshida, T. Yamaguchi, S. Kittaka, M.-C. Bellissent-Funel, P. Fouquet, D. Bowron
    • Organizer
      8th Liquid Matter Conference
    • Place of Presentation
      Wien (Austria)
    • Year and Date
      2011-09-08
  • [Presentation] Dynamics of Nano-confined Water2011

    • Author(s)
      K. Yoshida, T. Yamaguchi, S. Kittaka, M.-C. Bellissent-Funel, P. Fouquet
    • Organizer
      5th European Conference on Neutron Scattering
    • Place of Presentation
      Prague (Czech)
    • Year and Date
      2011-07-20

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Published: 2014-08-29  

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