2012 Fiscal Year Final Research Report
Development of fast and multi-functional biological analysis systembased on the ultra-micro droplet reaction place
| Project/Area Number |
22550079
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Tokyo Metropolitan University |
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Principal Investigator |
UCHIYAMA Katsumi 首都大学東京, 都市環境科学研究科, 教授 (40151899)
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| Project Period (FY) |
2010 – 2012
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| Keywords | マイクロチップ / 微少液滴 / 生化学分析 / インクジェット / マイクロ・ナノビーズ |
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| Research Abstract |
Micro-droplet reaction place was successfully developed by using ink-jet. Generation method of droplet from ink-jet was extensively analyzed and finally we have found the optimal conditions. By using droplet reaction system, size effect, which is the most important characteristics of micro-total analysis system, was realized. Using this droplet reaction system, measuring system for bio-samples using enzyme linked immune solvent assay was realized. The system for sample dispensing and measuring system for incident light fluorescent and chemiluminescence was developed. Then, three dimensional ordered structure with micro and nano beads was successfully developed and those structure were used for the novel analytical tool. ELISA using three dimensional ordered micro beads structure showed drastic increase in the detection sensitivity with 1/1000 reagent consumption when it was used as a reaction place. Finally, we have successfully fabricated photonic crystals with nano-bead and showed the possibility of material sensing by eyes.
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[Book] 基礎教育シリーズ,善木 道雄,寺前 紀夫,中釜 達朗,平山 和雄,三浦 恭之,南沢 宏明,森田 孝節分析化学〈基礎編〉2011
Author(s)
本水 昌二,磯崎 昭徳,櫻川 昭雄,井原 敏博,内山 一美,善木 道雄,寺前 紀夫,中釜 達朗,平山 和雄,三浦 恭之,南沢 宏明,森田 孝節
Total Pages
191
Publisher
株式会社東京教学社
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