2013 Fiscal Year Final Research Report
Precise hybrid synthesis of glycoprotein through amino acid sequence-specific introduction of oligosaccharide followed by enzymatic transglycosylation reaction
| Project/Area Number |
22550105
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Polymer chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
KOBAYASHI Atsushi 東北大学, 工学(系)研究科(研究院), 助教 (90361138)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | 糖タンパク質合成 / 酵素化学合成 / アマドリ転移反応 / 配列特異的反応 / 高分子反応 |
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| Research Abstract |
It is difficult to obtain homogeneous glycoproteins, although it is increasing our knowledge concerning physiological functions of glycoproteins. Nowadays, it is very easy to obtain bare proteins that fold appropriately by using prokaryote cells like Escherichia coli, and it is possible to obtain desired glycoproteins by conjugating with oligosaccharide and proteins precisely. Recently, it was reported that the Amadori rearrangement occurred between oligosaccharides and proteins in a sequence specific manner. Based on this report, I have investigated the effect of additives on the Amadori rearrangement reaction and acceleration of its reaction rate. The most striking result was that the effects of borate ion were depended upon chemical structure of sugars. For example, very large reaction rate using D-fructose was observed in phosphate buffer, although almost no reaction was observed in borate buffer. Investigation of sequence specificity of the Amadori rearrangement is under way.
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[Journal Article] Crystal structures of glycoside hydrolase family 51α-L-arabinofuranosidase from Thermotoga maritima2012
Author(s)
Do-Hyun. Im, Kei-ichi Kimura, Fumitaka Hayasaka, Tomonari Tanaka, Masato Noguchi, Atsushi Kobayashi, Shin-ichiro Shoda, Kentaro Miyazaki, Takayoshi Wakagi, and Shinya Fushinobu
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Journal Title
Biotechnology, and Biochemistry
Volume: 76
Pages: 423-42
DOI
Peer Reviewed
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[Journal Article] 4,6-Dimethoxy-1,3,5-triazin-2-ylβ-D-glycosaminides : Novel Substrates for Transglycosylation Reaction Catalyzed by Exo-β-D-glucosaminidase from Amycolatopsis orientalis2011
Author(s)
Tomonari Tanaka, Tomonori Wada, Masato Noguchi, Masaki Ishihara, Atsushi Kobayashi, Takayuki Ohnuma, Tamo Fukamizo, Ryszard Brzezinski, Shin-ichiro Shoda
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Journal Title
Journal of Carbohydrate Chemistry
Volume: 31
Pages: 634-646
DOI
Peer Reviewed
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[Journal Article] Efficient transfer of sialo-oligosaccharide onto proteins by combined use of a glycosynthase-like mutant of Mucor hiemalis endoglycosidase and synthetic sialo-complex-type sugar oxazoline2010
Author(s)
Midori Umekawa, T. Higashiyama, Y. Koga, T. Tanaka, M. Noguchi, Atsushi Kobayashi, S. Shoda, Wei Huang, Li-Xi Wang, Hisashi. Ashida, and Kenji Yamamoto
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Journal Title
Biochimica et Biophysica Acta, General Subjects
Volume: 1800
Pages: 1203-1209
DOI
Peer Reviewed
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[Journal Article] 4,6-Dimethoxy-1,3,5-triazine oligoxyloglucans : Novel one-step preparable substrates for studying action of endo-β-1,4-glucanase III from Trichoderma reesei2010
Author(s)
Atsushi Kobayashi, Tomonari Tanaka, K. Watanabe, Masaki Ishihara, Masato Noguchi, H. Okada, Yasushi Morikawa, and Shin-ichiro Shoda
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Journal Title
Bioorganic and Medicinal Chemistry Letters
Volume: 20
Pages: 3588-3591
DOI
Peer Reviewed
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