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2012 Fiscal Year Final Research Report

Structural studies on carbonyl sulfide hydrolase from Thiobacillus thioparus strain THI115

Research Project

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Project/Area Number 22550147
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemistry related to living body
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

NOGUCHI Keiichi  東京農工大学, 学術研究支援総合センター, 准教授 (00251588)

Co-Investigator(Renkei-kenkyūsha) ODAKA Masafumi  東京農工大学, 大学院・工学研究院, 准教授 (20224248)
Project Period (FY) 2010 – 2012
Keywords環境 / 金属酵素 / X線回折 / 結晶構造 / 触媒機構 / 基質認識
Research Abstract

Carbonyl sulfide is an atmospheric trace gas leading to sulfate aerosol formation, thereby participating in the global radiation balance and ozone chemistry. In this study, the crystal structure of carbonyl sulfide hydrolase (COSase) from Thiobacillus thioparus strain THI115 was determined to clarify its substrate recognition mechanism. Althoughthe overall structure including the catalytic site resembles those of the β-classcarbonic anhydrases, the α5 helix of COSase narrows its substrate pathway, which couldbe responsible for the substrate specificity.

  • Research Products

    (12 results)

All 2013 2012 2011 2010

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (11 results)

  • [Journal Article] Carbonyl sulfide hydrolase from Thiobacillus thioparus strain THI115 is one of the β-carbonic anhydrase family enzymes2013

    • Author(s)
      Ogawa T, Noguchi K, Saito M, Nagahata Y, Kato H, Ohtaki A, Nakayama H, Dohmae N, Matsushita Y, Odaka M, Yohda M, Nyunoya H, Katayama Y
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 135 Pages: 3818-3825

    • DOI

      DOI:10.1021/ja307735e.

    • Peer Reviewed
  • [Presentation] 活性化タンパク質P15Kによるチオシアネート加水分解酵素成熟化機構の解明2012

    • Author(s)
      渡邉俊則,他
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2012-12-16
  • [Presentation] 変異体を用いたチオシアネート加水分解酵素反応機構の解析2012

    • Author(s)
      渡邉俊則,他
    • Organizer
      酵素工学研究会第68回講演会
    • Place of Presentation
      東京大学山上会館
    • Year and Date
      2012-10-15
  • [Presentation] Functions of Two Strictly Conserved Arginine Residues in Fe-type Hydratase from Rhodococcus erythropolis N7712012

    • Author(s)
      相原敬之,他
    • Organizer
      生命医薬情報学連合大会
    • Place of Presentation
      タワーホール船堀
    • Year and Date
      2012-10-14
  • [Presentation] ニトリルヒドラターゼ触媒機構の時間分割X線結晶構造解析2012

    • Author(s)
      山中保明,他
    • Organizer
      第6回バイオ関連化学シンポジウム
    • Place of Presentation
      北海道大学
    • Year and Date
      2012-09-08
  • [Presentation] Difference in the substrate recognition among nitrile haydrate family2012

    • Author(s)
      相原敬之,他
    • Organizer
      12th Japan-China-Korea Joint Symposium on Enzyme Engineering
    • Place of Presentation
      金沢エクセルホテル東急
    • Year and Date
      2012-05-29
  • [Presentation] 基質アナログ複合体の結晶解析による硫化カルボニル分解酵素の基質認識機構の検討2011

    • Author(s)
      野口恵一,他
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学小金井キャンパス
    • Year and Date
      2011-09-26
  • [Presentation] Thiobacillus thioparus THI115由来硫化カルボニル分解酵素の触媒機構2011

    • Author(s)
      高橋翔平,他
    • Organizer
      第5回バイオ関連化学シンポジウム
    • Place of Presentation
      つくば国際会議場エポカルつくば
    • Year and Date
      2011-09-12
  • [Presentation] Carbonyl sulfide hydrolase: a novel β-carbonic anhydrase family enzyme catalyzing ambient level of carbonyl sulfide2011

    • Author(s)
      尾高雅文,他
    • Organizer
      15th International Conference on BiologicalInorganic Chemistry
    • Place of Presentation
      バンクーバー(カナダ)
    • Year and Date
      2011-08-07
  • [Presentation] 硫化カルボニル分解酵素の基質認識機構の解析2011

    • Author(s)
      野口恵一,他
    • Organizer
      第38回生体分子科学討論会
    • Place of Presentation
      筑波大学大学会館国際会議室
    • Year and Date
      2011-06-23
  • [Presentation] 硫化カルボニルを分解する新規酵素COSaseの構造化学的研究2010

    • Author(s)
      野口恵一
    • Organizer
      第23回生物無機化学夏季セミナー
    • Place of Presentation
      山梨県南都留郡鳴沢村森の家久野屋
    • Year and Date
      2010-08-30
  • [Presentation] Thiobacillus thioparus THI115の硫化カルボニル加水分解酵素(COSase)の酸素化学的性質および結晶構造解析2010

    • Author(s)
      小川貴弘,他
    • Organizer
      2010年度環境バイオテクノロジー学会
    • Place of Presentation
      宮城県東北大学片平さくらホール
    • Year and Date
      2010-06-22

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Published: 2014-08-29  

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