2012 Fiscal Year Final Research Report
Structural studies on carbonyl sulfide hydrolase from Thiobacillus thioparus strain THI115
| Project/Area Number |
22550147
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
NOGUCHI Keiichi 東京農工大学, 学術研究支援総合センター, 准教授 (00251588)
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| Co-Investigator(Renkei-kenkyūsha) |
ODAKA Masafumi 東京農工大学, 大学院・工学研究院, 准教授 (20224248)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 環境 / 金属酵素 / X線回折 / 結晶構造 / 触媒機構 / 基質認識 |
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| Research Abstract |
Carbonyl sulfide is an atmospheric trace gas leading to sulfate aerosol formation, thereby participating in the global radiation balance and ozone chemistry. In this study, the crystal structure of carbonyl sulfide hydrolase (COSase) from Thiobacillus thioparus strain THI115 was determined to clarify its substrate recognition mechanism. Althoughthe overall structure including the catalytic site resembles those of the β-classcarbonic anhydrases, the α5 helix of COSase narrows its substrate pathway, which couldbe responsible for the substrate specificity.
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[Journal Article] Carbonyl sulfide hydrolase from Thiobacillus thioparus strain THI115 is one of the β-carbonic anhydrase family enzymes2013
Author(s)
Ogawa T, Noguchi K, Saito M, Nagahata Y, Kato H, Ohtaki A, Nakayama H, Dohmae N, Matsushita Y, Odaka M, Yohda M, Nyunoya H, Katayama Y
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Journal Title
J. Am. Chem. Soc.
Volume: 135
Pages: 3818-3825
DOI
Peer Reviewed
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