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2012 Fiscal Year Final Research Report

Neutron structure analysis of human PGD synthase for elucidation of the reaction mechanism activated by metal ions

Research Project

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Project/Area Number 22550152
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemistry related to living body
Research InstitutionOsaka University

Principal Investigator

INOUE Tsuyoshi  大阪大学, 大学院・工学研究科, 教授 (20263204)

Co-Investigator(Kenkyū-buntansha) MATSUMURA Hiroyoshi  大阪大学, 大学院・工学研究科, 准教授 (30324809)
MIZOHATA Eiichi  大阪大学, 大学院・工学研究科, 助教 (90571183)
Project Period (FY) 2010 – 2012
Keywords中性子線解析 / 金属イオン / 酵素活性化機構
Research Abstract

Prostaglandin D_2(PGD_2) is an allergic and inflammatory mediator produced by hematopoietic PGDS (H-PGDS) in the presence of glutathione (GSH). Previously, we found that Ca^2+and Mg^2+activated the enzyme activity and uncovered the activation mechanism by the X-ray crystal structures of the Ca^2+- and Mg^2+-bound forms of human H-PGDS at high resolution. In this study, we performed the crystallization of H-PGDS to reveal the protonation state by neutron diffraction and obtained the crystals with maximum size of 0.5 mm^3with high quality.

  • Research Products

    (16 results)

All 2012 2011 2010 Other

All Journal Article (11 results) Presentation (5 results)

  • [Journal Article] Human hematopoietic prostaglandin D synthase inhibitor complex structures2012

    • Author(s)
      Y. Kado, Y. Okano, N. Okazaki, K. Aritake, N. Uodome, H. Matsumura, Y. Urade & T. Inoue
    • Journal Title

      J. Biochem

      Volume: 151 Pages: 447-455

  • [Journal Article] Three-dimensional, non-invasive, cross-sectional imaging of protein crystals using ultrahigh resolution optical coherence tomography2012

    • Author(s)
      N. Nishizawa, S. Ishida, M. Hirose, S. Sugiyama, T. Inoue, Y. Mori, K. Itoh & H. Matsumura*
    • Journal Title

      Biomed. Opt. Express

      Volume: 3 Pages: 735-740

  • [Journal Article] Growth of protein crystals in hydrogels prevents osmotic shock2012

    • Author(s)
      S. Sugiyama, M. Maruyama, G. Sazaki, M. Hirose, H. Adachi, K. Takano, S. Murakami, T. Inoue, Y. Mori & H. Matsumura*
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 134 Pages: 5786-5789

  • [Journal Article] Crystal structure of the superfamily 1 helicase from Tomato mosaic virus2012

    • Author(s)
      M. Nishikiori, S. Sugiyama, H. Xiang, M. Niiyama, K. Ishibashi, T. Inoue, M. Ishikawa, H. Matsumura & E. Katoh*
    • Journal Title

      J. Virol.

      Volume: 86 Pages: 7565-7576

  • [Journal Article] Effects of a forced solution flow on the step advancement on {110} faces of tetragonal lysozyme crystals: direct visualization of individual steps under a forced solution flow2012

    • Author(s)
      M. Maruyama, K. Hisato, G. Sazaki, S. Maki, Y. Takahashi, H. Yoshikawa, S. Sugiyama, H. Adachi, K. Takano, H. Matsumura, T. Inoue, S. Murakami & Y. Mori
    • Journal Title

      Cryst. Growth Des.

      Volume: 12 Pages: 2856-2863

  • [Journal Article] The in silico screening and X-ray structure analysis of the inhibitor complex of Plasmodium falciparum orotidine 5'-monophosphate decarboxylase2012

    • Author(s)
      Y. Takashima, E. Mizohata, S. R. Krungkrai, Y. Fukunishi, T. Kinoshita, T. Sakata, H. Matsumura, J. Krungkrai, T. Horii & T. Inoue
    • Journal Title

      J. Biochem.

      Volume: 152 Pages: 133-138

  • [Journal Article] Crystal structure of the N-terminal domain of the yeast general corepressor Tup1p and its functional implications2012

    • Author(s)
      H. Matsumura*, N. Kusaka, T. Nakamura, N. Tanaka, K. Sagegami, K. Uegaki, T. Inoue & Y. Mukai
    • Journal Title

      J. Biol. Chem.

      Volume: 287 Pages: 26528-26538

  • [Journal Article] Crystal structure of rice Rubisco and implications for activation induced by positive effectors NADPH and 6-phosphogluconate2012

    • Author(s)
      H. Matsumura, E. Mizohata, H. Ishida, A. Kogami, T. Ueno, A. Makino, T. Inoue, A. Yokota, T. Mae & Y. Kai*
    • Journal Title

      J. Mol. Biol.

      Volume: 422 Pages: 75-86

  • [Journal Article] Spatially precise, soft microseeding of single protein crystals by femtosecond laser ablation2012

    • Author(s)
      H. Yoshikawa, Y. Hosokawa, R. Murai, G. Sazaki, T. Kitatani, H. Adachi, T. Inoue, H. Matsumura, K. Takano, S. Murakami, S. Nakabayashi, Y. Mori & H. Matsumura
    • Journal Title

      Cryst. Growth Des.

      Volume: 12 Pages: 4334-4339

  • [Journal Article] Structural insight into the stereoselective production of PGF_2αby Old Yellow Enzyme from Trypanosoma cruzi2011

    • Author(s)
      N. Okamoto, K. Yamaguchi, E. Mizohata, K. Tokuoka, N. Uchiyama, S. Sugiyama, H. Matsumura, K. Inaka, Y. Urade, T. Inoue
    • Journal Title

      J. of Biochemistry

      Volume: 150 Pages: 563-568

  • [Journal Article] Crystallization and preliminary X-ray diffraction analysis of mouse prostaglandin F_2αsynthase, AKR1B32011

    • Author(s)
      Y. Takashima, S. Hatanaka, E. Mizohata, N. Nagata, Y. Fukunishi, H. Matsumura, Y. Urade, T. Inoue
    • Journal Title

      Acta Cryst.

      Volume: F67 Pages: 1630-1632

  • [Presentation] Atomic resolution structure of copper-containing nitrite reductase provides insights into common properties of type 2 copper-containing enzymes2012

    • Author(s)
      Y. Fukuda, T. Tamada, H. Takami, T. Inoue, M. Nojiri
    • Organizer
      Asian Crystallographic Association (AsCA) 2012
    • Place of Presentation
      Adelaide (Australia)
    • Year and Date
      20121202-05
  • [Presentation] Structural basis enzymes in the pyrimidine biosynthetic pathway of human malaria parasite Plasmodium falciparumfor in silicodrug design2011

    • Author(s)
      Y. Takashima, E. Mizohata, K. Tokuoka, Y. Kusakari, T. Uchida, S. R. Krungkrai, H. Matsumura, J. Krungkrai, T. Horii, T. Inoue
    • Organizer
      International Union of Crystallography
    • Place of Presentation
      Madrid(Spain)
    • Year and Date
      20110822-30
  • [Presentation] Novel Approach for Growth of High-Quality and Large Protein Crystals2010

    • Author(s)
      H. Matsumura, K. Kakinouchi, N. Shimizu, S. Sugiyama, Y. Takahashi, M. Maruyama, R. Murai, H. Adachi, K. Takano, S. Murakami, T. Inoue, Y. Mori
    • Organizer
      3th International Conference on the Crystallization of Biological Macromolecules
    • Place of Presentation
      Dublin(Ireland)
    • Year and Date
      20100912-16
  • [Presentation] Protein Crystallization in Agarose Gel with High Strength2010

    • Author(s)
      S. Sugiyama, M. Hirose, R. Murai, Y. Takahashi, M. Maruyama, G. Sazaki, H. Adachi, K. Takano, S. Murakami, T. Inoue, H. Matsumura
    • Organizer
      3th International Conference on the Crystallization of Biological Macromolecules
    • Place of Presentation
      Dublin(Ireland)
    • Year and Date
      20100912-16
  • [Presentation] Development of the new protein crystallization techniques and application for structural analysis of target Enzymes

    • Author(s)
      T. Inoue, H. Adachi, K. Takano, H. Matsumura, S. Murakami, Y. Mori
    • Organizer
      The 10th KIAS Conference on Protein Structure for structural and Function
    • Place of Presentation
      Korea Institute for Advanced Study, Seoul, Korea

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Published: 2014-08-29  

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