2012 Fiscal Year Final Research Report
Development of fluorometric potassium ion imaging reagent in a cell
| Project/Area Number |
22550154
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Kyushu Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Keywords | ナノバイオ / 分子認識 / 細胞・組織 / 生体分子 / 蛍光プローブ |
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| Research Abstract |
In this project, we successful synthesized a fluorometric potassiumdetecting reagent, PSO-5, consisted of thrombin binding aptamer (TBA) carrying FAM at the 5'-end conjugated with a peptide carrying TAMRA and biotin at the middle and at its C-terminus, respectively. K^+ in the cell was visualized based on the FRET ratio of the ternary complex of streptavidin with PSO-5 and biotinylated nuclear export signal peptide. Change of FRET signal of PSO-5 was observed that indicated a decrease in K^+ concentration in the cell upon addition of apoptosis-inducing drug to monitor K^+ efflux from the cell.
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