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2012 Fiscal Year Final Research Report

y -Cyclodextrin-polyurethane copolymer adsorbent for selective removalof endotoxin from DNA solution

Research Project

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Project/Area Number 22550200
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Polymer/Textile materials
Research InstitutionKumamoto University

Principal Investigator

SAKATA Masayo  熊本大学, 大学院・自然科学研究科, 准教授 (60187391)

Co-Investigator(Renkei-kenkyūsha) SASAKI Mitsuru  熊本大学, 大学院・自然科学研究科, 准教授 (40363519)
Project Period (FY) 2010 – 2012
Keywords高分子微粒子 / エンドトキシン / LPS 吸着剤 / DNA 分離 / 包接作用 / 選択吸着
Research Abstract

The copolymer particles for removal of lipopolysaccharide (LPS)were prepared by suspension copolymerization of -cyclodextrin (CyD) and1,6-hexamethylenediisocyanate. The copolymer (CyD content: 14-20 mol%) could selectivelyremoved LPS from a DNA solution (50 g/ml) containing LPS (15 EU/ml) at pH 6 and ionicstrength (u) of 0.05-0.2.

  • Research Products

    (27 results)

All 2013 2012 2011 2010 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (16 results) Book (1 results) Remarks (4 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Immobilization of trypsin on grapheneoxide nanosheets for increasedproteolytic stability2012

    • Author(s)
      M. Sakata, A. Funatsu, S. Sonoda, T.Ogata, T. Taniguchi, Y. Matsumoto
    • Journal Title

      Chemstry letters

      Volume: Vol.41, No.12 Pages: 1625-1627

    • Peer Reviewed
  • [Journal Article] Photochemicalengineering of graphene oxide nanosheets2012

    • Author(s)
      M. Koinuma, C. Ogata, Y. Kamei, K.Hatakeyama, H. Tateishi, Y. Watanabe, T.Taniguchi, K. Gezuhara, S. Hayami, A.Funatsu, M. Sakata, Y. Kawahara, S.Kurihara, Y. Matsumoto
    • Journal Title

      Journal of Physical Chemistry C

      Volume: Vol.116, No.37 Pages: 19822-19827

    • Peer Reviewed
  • [Journal Article] Selectiveremoval of endotoxin from a DNA solutionby cross-linked cyclodextrin beads2011

    • Author(s)
      M. Sakata, K. Yoshimura, I. Sakamoto,M. Todokoro, M. Kunitake
    • Journal Title

      Analyrical Sciences

      Volume: Vol. 27 Pages: 213-216

    • Peer Reviewed
  • [Journal Article] Selective Removal of Glucoamylase from unpasteurized sake materials

    • Author(s)
      M. Sakata, F. Kurogi, K. Kai, M.Kunitake
    • Journal Title

      Journal of Liquid Chromatography & RelatedTechnologies

      Volume: (印刷中)

    • DOI

      DOI:10.1080/10826076.2012.692145

    • Peer Reviewed
  • [Presentation] 酵素修飾ナノシートの調製とその酵素活性維持能の評価2013

    • Author(s)
      坂田眞砂代、 船津麻美、 園田将平、 松本泰道
    • Organizer
      日本化学会第93 春季年会
    • Place of Presentation
      立命館大学びわこくさつキャンパス (草津市滋賀県)
    • Year and Date
      2013-03-23
  • [Presentation] r-Cyclodextrin-polyurethanecopolymer adsorbent for selectiveremoval of endotoxin from DNA solution2012

    • Author(s)
      M. Sakata, K. Uezono, K. Kimura, M.Todokoro
    • Organizer
      3rd International Cellulose Conference
    • Place of Presentation
      Chateraise Gateaux KingdomSapporo Hotel & SPA Resort (Sapporo,Japan)
    • Year and Date
      2012-10-11
  • [Presentation] Design of cellulose beadsgrafted with cationic polymer forchromatographic separation of DNAfrom protein solution2012

    • Author(s)
      Y. Goto , E. Ikegami, Y. Tatenaka, M.Sakata
    • Organizer
      3rd International Cellulose Conference
    • Place of Presentation
      Chateraise Gateaux KingdomSapporo Hotel & SPA Resort (Sapporo,Japan)
    • Year and Date
      2012-10-11
  • [Presentation] DNA 溶液からのエンドトキシン選択除去のためのシクロデキストリン-ウレタン共重合粒子2012

    • Author(s)
      木村かさね、上園康史、戸所正美、坂田眞砂代
    • Organizer
      第61回高分子討論会
    • Place of Presentation
      名古屋工業大学 (名古屋市)
    • Year and Date
      2012-09-19
  • [Presentation] Chromatographic separationof DNA from protein solution bycellulose beads grafted with cationicpolymer chains through ATRP2012

    • Author(s)
      M. Sakata, Y. Goto, Y. Tatenaka, K.Ishikura
    • Organizer
      38thInternational Symposium on HighPerformance Liquid Phase Separationsand Related Techniques
    • Place of Presentation
      Anaheim Marriott, (California, USA)
    • Year and Date
      2012-06-19
  • [Presentation] シクロデキストリン架橋粒子による DNA 溶液からの内毒素のクロマト分離2012

    • Author(s)
      上園康史、吉村佳奈、國武雅司、坂田眞砂代
    • Organizer
      第26回産学官技術交流会
    • Place of Presentation
      第26回産学官技術交流会
    • Year and Date
      2012-01-18
  • [Presentation] Chromatographic removal ofendotoxin from DNA solution bycross-linked cyclodextrin beads2011

    • Author(s)
      K. Uezono, K. Yoshimura, M. Sakata, M.Kunitake
    • Organizer
      The12TH Pacific Polymer Conference
    • Place of Presentation
      The Shilla Hotel (JejuIsland, Korea)
    • Year and Date
      2011-11-14
  • [Presentation] Cross-linked cyclodextrinbeads for chromatographic removal ofendotoxin from DNA solution2011

    • Author(s)
      M. Sakata, K. Uezono, K.Yoshimura, M.Kunitake
    • Organizer
      The 3rd Asian Symposium on Advanced Materials
    • Place of Presentation
      九州大学 (福岡)
    • Year and Date
      2011-09-20
  • [Presentation] r-シクロデキストリン架橋粒子を用いた DNA 溶液からのエンドトキシンの除去2011

    • Author(s)
      上園康史、吉村佳奈、坂田眞砂代、國武雅司
    • Organizer
      第48回化学関連支部合同九州大会
    • Place of Presentation
      北九州国際会議場 (北九州市)
    • Year and Date
      2011-07-09
  • [Presentation] DNA クロマト分離に及ぼすアミノ化セルロース粒子のリガンド密度とポアサイズの影響2011

    • Author(s)
      五島裕介、立中佑希、坂田眞砂代、國武雅司
    • Organizer
      第48回化学関連支部合同九州大会
    • Place of Presentation
      北九州国際会議場(北九州市)
    • Year and Date
      2011-07-09
  • [Presentation] Removal of endotoxin fromDNA solution by cyclodextrin polymerbeads2011

    • Author(s)
      M. Sakata, K. Yoshimura, K. Uezono, M.Kunitake
    • Organizer
      The 2nd FAPS Polymer Congress
    • Place of Presentation
      China National ConventionCenter (Beijing, China)
    • Year and Date
      2011-05-10
  • [Presentation] DNA水溶液からの LPS 除去のためのシクロデキストリン架橋粒子の調製2010

    • Author(s)
      吉村佳奈、坂本逸美、坂田眞砂代
    • Organizer
      第59回高分子学会年次大会
    • Place of Presentation
      パシフィコ横浜,横浜市
    • Year and Date
      20100526-28
  • [Presentation] Design of cellulose beads grafted withcationic polymer for chromatographicseparation of DNA from proteinsolution2010

    • Author(s)
      M.Sakata, Y. Tatenaka, Md.Ashaduzzaman, K. Ishikura, M. Kunitake
    • Organizer
      The 2010 InternationalChemical Congress of Pacific BasinSocieties
    • Place of Presentation
      Convention Center (Honolulu, Hawaii, U.S.A)
    • Year and Date
      2010-12-16
  • [Presentation] LPS 選択吸着除去のためのシクロデキストリン架橋粒子の設計とその応用2010

    • Author(s)
      吉村佳奈,坂本逸美,上園康史,坂田眞砂代,國武雅司
    • Organizer
      2010 年日本化学会西日本大会
    • Place of Presentation
      熊本大学 (熊本市)
    • Year and Date
      2010-11-06
  • [Presentation] Chromatographic separation of DNA fromprotein solution by cellulose beadsgrafted with cationic polymer chainsthrough ATRP2010

    • Author(s)
      Md. Ashaduzzaman, Y. Tatenaka, K.Ishikura, M. Sakata, M. Kunitake
    • Organizer
      10th InternationalConference on Fundamentals ofAdsorption
    • Place of Presentation
      AwajiYumebutai International ConferenceCenter (Awaji, Japan)
    • Year and Date
      2010-05-25
  • [Presentation] Novel endotoxin assay byadsorption method withpolycation-immobi-lized cellulosebeads and limulus amoebocyte lysate2010

    • Author(s)
      M. Sakata, T. Ogata, M. Todokoro, M.Kunitake
    • Organizer
      10th International Conference onFundamentals of Adsorption
    • Place of Presentation
      Awaji Yumebutai InternationalConference Center (Awaji, Japan)
    • Year and Date
      2010-05-24
  • [Book] Biopolymers: Separations. Cazes(EDT)2010

    • Author(s)
      Masayo Sakata, Chuichi Hirayama
    • Total Pages
      268-273
    • Publisher
      Encyclopedia of Chromatography (3rdEdition)
  • [Remarks]

    • URL

      http://www.kumamoto-u.ac.jp/daigakujouhou/...file/release121019-2.pdf

  • [Remarks]

    • URL

      http://www.spsj.or.jp/koho/21PMF/21PMF_4.pdf

  • [Remarks] DNA ワクチンの製造時、有害物質除く吸着剤、日本経済新聞, 2012.10.25, 35 面

  • [Remarks] ワクチンから有毒物質除去、熊本日日新聞,2012.10.31, 32 面

  • [Patent(Industrial Property Rights)] エンドトキシン吸着材2012

    • Inventor(s)
      坂田 眞砂代、戸所 正美
    • Industrial Property Rights Holder
      熊本大学、JNC 株式会社
    • Industrial Property Number
      2012-123281
    • Filing Date
      2012-05-30
  • [Patent(Industrial Property Rights)] エンドトキシン吸着体、 及びそれを用いたエンドトキシンの除去方法2012

    • Inventor(s)
      戸所正美、平山忠一、國武雅司、坂田眞砂代
    • Industrial Property Rights Holder
      チッソ株式会社
    • Industrial Property Number
      2012-4996791
    • Acquisition Date
      2012-05-18

URL: 

Published: 2014-08-29  

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