2012 Fiscal Year Final Research Report
Assessment of crystal quality of protein crystals by using rocking curve imaging.
| Project/Area Number |
22560661
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical properties of metals
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| Research Institution | Yokohama Soei Junior College |
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Principal Investigator |
KOJIMA Kenichi 横浜創英短期大学, 情報学科, 教授 (90046095)
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| Co-Investigator(Kenkyū-buntansha) |
WAKO Kei 横浜創英短期大学, 情報学科, 講師 (40515839)
TACHIBANA Masaru 横浜市立大学, 生命ナノシステム科学研究科, 教授 (80236546)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 格子欠陥 / 転位 / 放射光 X 線回折トポグラフ / ロッキングカーブ / イメイジング / デジタルトポグラフ |
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| Research Abstract |
In the past, the main goal of protein crystallization was to explore the structure of protein molecules using X-ray. Recently, protein crystals have emerged as promising bio-nanoporous materials for different applications including highly selective bio-catalysis, bio-sensing and drug delivery. We identified the dislocation images in the identical protein crystals which are tetragonal hen-egg white lysozyme (HEWL) crystals not only by the conventional film method but the digital method using X-ray CCD camera systems. The simultaneous measurements of both methods were also carried out in the identical HEWL crystals. We evaluate the crystal perfection by mapping angular positions of maximum and the full width of half maximum (FWHM) for the local rocking curves. It is shown that the digital topography is more effective for assessment of crystal quality in protein crystals.
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