2012 Fiscal Year Final Research Report
Development of Purification Process of Plasmid DNA for Gene Therapy Using Affinity Membrane Filtration
| Project/Area Number |
22560746
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Properties in chemical engineering process/Transfer operation/Unit operation
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IRITANI Eiji 名古屋大学, 大学院・工学研究科, 教授 (60144119)
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| Project Period (FY) |
2010 – 2012
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| Keywords | プラスミドDNA / アフィニティ / 膜濾過 / リガンド / 吸着 / 脱着 / 精製 / 透過 |
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| Research Abstract |
Recently, the use of plasmid DNA as vector for gene therapy or DNA vaccination has become increasingly popular as novel ways of dealing with disease. For such clinical applications, there is a growing need for purification processes to produce pharmaceutical-grade plasmid DNA. In the present study, the application of affinity membrane filtration to plasmid DNA purification and the search of ligand are examined. In addition to the selectivity behaviors in the binding process of plasmid DNA to ligand and the elution process of the bound plasmid DNA, the flux decline behaviors caused by the membrane fouling are also investigated in this system. The agarose gel analysis confirmed usefulness of this method for plasmid DNA purification.
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