2012 Fiscal Year Final Research Report

Development of Site-Directed Immobilization Method to ConstructHigh-Efficient Bioreactors

Research Project

Project/Area Number	22560771
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Biofunction/Bioprocess
Research Institution	Chubu University (2011-2012) Okayama University (2010)
Principal Investigator	NAKANISHI Kazuhiro 中部大学, 応用生物学部, 教授 (90026584)
Co-Investigator(Kenkyū-buntansha)	IMAMURA Koreyoshi 岡山大学, 自然科学研究科, 教授 (70294436) IMANAKA Hiroyuki 岡山大学, 自然科学研究科, 助教 (10379711)
Project Period (FY)	2010 – 2012
Keywords	酵素 / 配向制御固定化 / 親和性ペプチドタグ / シリカ / ポリスチレン
Research Abstract	In this study, a method to immobilize enzymes/proteins on the solid surface without suffering from denaturation that might occur upon coming in contact with the solid surface was investigated. Conjugating peptide tags that show a high affinity to silica and polystyrene surfaces, toenzymes enabled a strong, nearly irreversible immobilization without appreciable loss of activity.