Spatiotemporal alteration of chromatin structure at promoter regions

2012 Fiscal Year Final Research Report

• Project/Area Number: 22570006
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: Fujita Health University
• Principal Investigator: ISHIHARA Naoe 藤田保健衛生大学, 医学部, 研究員 (50571791)
• Co-Investigator(Kenkyū-buntansha): ISHIHARA Satoru 藤田保健衛生大学, 医学部, 講師 (00300723)
• Project Period (FY): 2010 – 2012
• Keywords: アロマターゼ / クロマチン / ヒストン / プロモーター / 転写調節

Research Abstract

We analyzed chromatin structure responsible to the alternativepromoter usage of the aromatase gene (CYP19A1), which is transcribed from three majorpromoters. Our novel method using sedimentation velocity centrifugation revealed thatworking promoters were exclusively composed of open chromatin. In contrast, therecruitment of trimethylation of histone H3 lysine 27, which is known as a negative markfor transcription, was observed around unused promoters, suggesting that polycombproteins repressed the promoters selectively.

Research Products

• [Journal Article] The role of IL-15 in activating STAT5 and fine-tuning IL-17A production in CD4 T lymphocytes (2012)
  • Author(s): Pandiyan,P., Yang,XP., Saravanamuthu, SS., Zheng,L., Ishihara,S., O'Shea,JJ., and Lenardo, MJ
  • Journal Title: J. Immunol Volume: 189 Pages: 4237-4246
  • DOI: doi:10.4049/jimmunol.1201476
  • Peer Reviewed
• [Journal Article] Complex cell cycle abnor- malities caused by human T-lympho-tropic virus type 1 Tax. (2011)
  • Author(s): Yang, L., Kotomura, N., Ho,YK., Zhi, H., Bixler, S., Schell, MJ.,and Giam,CZ
  • Journal Title: J. Virol Volume: 85 Pages: 3001-3009
  • DOI: doi:10.1128/JVI.00086-10
  • Peer Reviewed
• [Journal Article] Two-step binding of transcriptionfactors causes sequential chromatinstructural changes at the activatedIL-2 promoter (2011)
  • Author(s): Ishihara, S. and Schwartz, RH
  • Journal Title: J. Immunol Volume: 187 Pages: 3292-3299
  • DOI: doi:10.4049/jimmunol.1003173
  • Peer Reviewed
• [Journal Article] A new fractionation assay,based on the size of formaldehydecrosslinked, mildly sheared chroma-tin, delineates the chromatin struc-ture at promoter regions (2010)
  • Author(s): Ishihara, S., Varma, R., and Schwartz,RH
  • Journal Title: NucleicAcids Res Volume: 38 Pages: e124
  • DOI: doi:10.1093/nar/gkq203
  • Peer Reviewed
• [Presentation] The chromatin structure responsible for the degree of the promoter activity (2012)
  • Author(s): 琴村直恵,原田信広,石原悟
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 20121211-14
• [Presentation] CYP19遺伝子のプロモーター選択機構:密なクロマチン構造でプロモーターを選択的に抑制する (2012)
  • Author(s): 石原悟,琴村直恵,原田信広
  • Organizer: 第6回日本エピジェネティクス研究会年会
  • Place of Presentation: 東京
  • Year and Date: 20120514-15
• [Presentation] Thecontribution of histone modifica-tions to alternative promoter usageof the human CYP19 gene. (2011)
  • Author(s): 琴村直恵,原田信広,石原悟
  • Organizer: 第34回日本分子生物学会年会.
  • Place of Presentation: 横浜.
  • Year and Date: 20111213-16
• [Presentation] Condensed Chromatin Marked by K27-Trimethy-lated Histone H3 Determines Alterna-tive Promoter Usage of the CYP19 Gene (2011)
  • Author(s): 石原悟,琴村直恵,原田信広
  • Organizer: 第5回日本エピジェネティクス研究会年会
  • Place of Presentation: 熊本
  • Year and Date: 20110519-20
• [Presentation] Condensed chromatin marked by K27-trimethylated histone H3 determinesalternative promoter usage of the CYP19 gene (2011)
  • Author(s): Kotomura,N.,Harada,N.,Ishihara,S
  • Organizer: CDB Symposium"Epigene- tic landscape in development and dis-ease
  • Place of Presentation: Kobe,JAPAN
  • Year and Date: 20110314-15
• [Presentation] ヒストンH3K27のトリメチル化によるアロマターゼ遺伝子プロモーターの選択的不活性化 (2011)
  • Author(s): 石原悟,琴村直恵,西芳寛,野村政壽,柳瀬敏彦,原田信広
  • Organizer: 第19回日本ステロイドホルモン学会学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2011-11-26
• [Presentation] Sequentialrearrangement and eviction of nucleo-somes allow interleukin-2 transcrip-tion following T cell activation. (2010)
  • Author(s): Ishihara,S.,Schwartz,RH
  • Organizer: TheNIBB Conference " DYNAMICGENOME "
  • Place of Presentation: Okazaki, JAPAN
  • Year and Date: 2010-10-16