Study on mitochondrial bottleneck and diseases

2012 Fiscal Year Final Research Report

• Project/Area Number: 22570009
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: 公益財団法人東京都医学総合研究所 (2012); The Institute of Physical and Chemical Research (2010-2011)
• Principal Investigator: SOU Reikin 公益財団法人東京都医学総合研究所, 基盤技術研究センター, 研究員 (60399475)
• Project Period (FY): 2010 – 2012
• Keywords: ミトコンドリア / ボトルネック / ミトコンドリア分離 / 分子遺伝 / 遺伝病 / 生体イメージング

Research Abstract

This study aims to elucidate the mechanism of mtDNA disease transmission between generations using mouse strains heteroplasmic for two mtDNA variants, and understand alterations of mitochondrial dynamics in live mouse internal organs in disease and stress conditions using intravital imaging technology. We have identified two heteroplasmic mouse strains suitable for mtDNA transmission analysis and established the methods to precisely examine the ratio of two mtDNA variants in single cells. Further, we have invented a stabilization device which effectively suppresses the tissue motion of abdominal organs and monitored mitochondrial dynamics in various intraperitoneal organs in live mice.

Research Products

• [Journal Article] High resolution intravital imaging of subcellular structures of mouse abdominal organs using a microstage device (2012)
  • Author(s): Cao, L., Kobayakawa S., Yoshiki A., and Abe K
  • Journal Title: PLoS One Volume: 7 Pages: e33876
  • Peer Reviewed
• [Journal Article] The mode of mitochondrial DNA transmission in mammals: rapid segregation and bottleneck effect (2010)
  • Author(s): Shitara, H., Cao L., and Yonekawa H
  • Journal Title: Cell Technology Volume: 29 Pages: 461-465
• [Presentation] In vivo imaging of subcellular structures, mitochondria and autophagosomes, in live mouse abdominal organs using a microstage device (2012)
  • Author(s): Cao, L. and Abe K
  • Organizer: The 12^<th> Conference of Japanese Society of Mitochondrial Research and Medicine
  • Place of Presentation: Tsukuba, Ibaraki, Japan
  • Year and Date: 20121219-21
• [Presentation] High resolution intravital imaging of subcellular structures of mouse abdominal organs using a microstage device (2012)
  • Author(s): Cao, L., Kobayakawa S., Yoshiki A., and Abe K
  • Organizer: The 26^<th> Molossinus Symposium
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20120515-16
• [Presentation] Solid evidence proves that the mitochondrial bottleneck occurs without reduction of mitochondrial DNA molecules in early primordial germ cells of mice (2011)
  • Author(s): Cao, L., Shitara H., Sugimoto M., Hayashi J.-I., Abe K., Yonekawa H
  • Organizer: The eighth European Meeting on Mitochondrial Pathology (Euromit 8)
  • Place of Presentation: Zaragoza, Spain
  • Year and Date: 20110620-23
• [Presentation] Intravital molecular imaging of cellular processes in live mice (2010)
  • Author(s): Cao, L., and Abe K. (invited speaker)
  • Organizer: The 4^<th> congress of Asia federation of laboratory animal science
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 20101109-11
• [Presentation] The mitochondrial DNA bottleneck in the germline cells of mammals (2010)
  • Author(s): Cao, L., Shitara H., Sugimoto M., Hayashi J.-I., Abe K., and Yonekawa H. (invited speaker)
  • Organizer: Eastern Japanese animal embryo transfer workshop and animal genetics and reproduction seminar
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2010-12-13