2013 Fiscal Year Final Research Report
Analysis of bacterial-type plastidial RNA binding protein required for chloroplast development
| Project/Area Number |
22570045
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Kyushu University |
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Principal Investigator |
KUSUMI Kensuke 九州大学, 理学(系)研究科(研究院), 助教 (00304725)
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| Project Period (FY) |
2010-10-20 – 2013-03-31
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| Keywords | 色素体機能 / 光合成 / 転写装置 / 翻訳装置 / RNA結合タンパク質 / rRNA / 葉緑体分化 |
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| Research Abstract |
NUS1 protein is plastidial RNA binding protein essential for chloroplast biogenesis under low temperature. NUS1 is structurally similar to the bacterial protein NusB required for rRNA expression. NUS1 protein accumulated abundantly in the pre-emerged immature leaf whereas it disappeared in the mature leaf. NUS1 has a molecular weight of 30 kDa, and the BN-PAGE analyses showed that NUS1 was involved in the ~110kDa protein complex. Mobility gel shift assays indicated that NUS1 protein bound to 5' precursor region of chloroplast 16S rRNA. NUS1-deficient mutant showed severe leaf chlorosis at low temperature. In the chlorotic leaves of the mutant seedling, maturation of chloroplast rRNA was impaired and the chloroplast translation/transcription capacity was severely suppressed, suggesting that NUS1 has important roles in the regulation of chloroplast genetic system during early leaf development in rice.
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[Journal Article] Diversity in Guanosine 3',5'-Bisdiphosphate (ppGpp) Sensitivity Among Guanylate Kinases of Bacteria and Plants2014
Author(s)
Nomura, Y., Izumi, A., Fukunaga, Y., Kusumi, K., Iba, K., Watanabe, S., Nakahira, Y., Weber, A.P., Nozawa, A., and Tozawa, Y.
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Journal Title
DOI
Peer Reviewed
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[Journal Article] A Dof Transcription Factor, SCAP1, Is Essential for the Development of Functional Stomata in Arabidopsis2013
Author(s)
Negi, J., Moriwaki, K., Konishi, M., Yokoyama, R., Nakano, T., Kusumi, K., Hashimoto-Sugimoto, M., Schroeder, J.I., Nishitani, K., Yanagisawa, S., and Iba, K.
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Journal Title
Current biology
Volume: CB 23
Pages: 479-484
DOI
Peer Reviewed
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[Journal Article] Interaction between a plasma membrane-localized ankyrin-repeat protein ITN1 and a nuclear protein RTV12012
Author(s)
Sakamoto, H., Sakata, K., Kusumi, K., Kojima, M., Sakakibara, H., and Iba, K.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 423
Pages: 392-397
DOI
Peer Reviewed
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[Journal Article] A plastid protein NUS1 is essential for build-up of the genetic system for early chloroplast development under cold stress conditions2011
Author(s)
Kusumi, K., Sakata, C., Nakamura, T., Kawasaki, S., Yoshimura, A., and Iba, K.
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Journal Title
Plant J.
Volume: 68
Pages: 1039-1050
DOI
Peer Reviewed
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[Journal Article] Environmental regulation of stomatal response in the Arabidopsis Cvi-0 ecotype2011
Author(s)
Monda, K., Negi, J., Iio, A., Kusumi, K., Kojima, M., Hashimoto, M., Sakakibara, H., and Iba, K.
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Journal Title
Planta
Volume: 234
Pages: 555-563
DOI
Peer Reviewed
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