2012 Fiscal Year Final Research Report

Function of D-amino acids on plastid division in plants

Research Project

Project/Area Number	22570046
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Plant molecular biology/Plant physiology
Research Institution	Kumamoto University
Principal Investigator	TAKANO Hiroyoshi 熊本大学, バイオエレクトリクス研究センター, 教授 (70242104)
Co-Investigator(Kenkyū-buntansha)	TAKACHI Katsuaki 熊本大学, 大学院・自然科学研究科, 准教授 (70515501)
Project Period (FY)	2010 – 2012
Keywords	D-アミノ酸 / コケ植物 / 葉緑体分裂 / オルガネラ
Research Abstract	It is thought that usage of D-amino acids in organisms is restrictive, although D-aminoacids are essential components of bacterial peptidoglycan. We found that plastidpeptidoglycan synthesis pathway remains in the moss Physcomitrella patens. In this study,we analyzed a P. patens homolog of bacterial D-alanine-D-alanine ligase (Ddl). Wegenerated a PpDdl knockout line, and its phenotype showed a few macrochloroplasts.D-alanyl-D-alanine supplemented in a medium could suppress chloroplast phenotype. Theseresults suggested that D-alanyl-D-alanine synthesized with PpDdl is essential forchloroplast division in P. patens.