2012 Fiscal Year Final Research Report
Application of gene targeting by homologous recombination as a biothecnological tool for rice functional genomics.
Project/Area Number |
22570049
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | University of Shizuoka |
Principal Investigator |
JOHZUKA Yasuyo 静岡県立大学, 融合科学研究科(研究院), 博士研究員 (60517887)
|
Co-Investigator(Kenkyū-buntansha) |
NOGUCHI Hiroshi 静岡県立大学, 薬学部, 教授 (60126141)
IIDA Shigeru 静岡県立大学, 大学院・生活科学健康科・薬学研究科, 特任教授 (30012777)
|
Project Period (FY) |
2010 – 2012
|
Keywords | 遺伝子ターゲティング / 相同組換え / 変異導入 / 植物 / イネ / 形質転換 / バイオテクノロジー / 遺伝子 |
Research Abstract |
Rice, an important food, is the staple grain for one-half of the world’s population. We have succeeded in the development of a gene-targeting method which involves the genetic modification of rice DNA. Aiming at wide use of targeting of a higher plant, we developed making suitable recombination enzyme (λRed system) , and making targeting frequency increase. The vectors for assay for measuring targeting frequency were also developed.
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