Epigenetics during pollination and fertilization in higher plants

2012 Fiscal Year Final Research Report

• Project/Area Number: 22570068
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Yokohama City University
• Principal Investigator: TANAKA Ichiro 横浜市立大学, 大学院・生命ナノシステム科学研究科, 教授 (60175445)
• Project Period (FY): 2010 – 2012
• Keywords: 精細胞 / 花粉 / 花粉管 / ヒストン / 雄原細胞 / 雄性配偶子

Research Abstract

In this study, we first showed that Argonaute (AGO) was specifically expressed in the generative and sperm cells in Lilium longiflorum. This suggests that the gene expression in male gametic cells might be regulated epigenetically. Futher, we found dimorphism between two sperm cells in the nuclear chromatin

Research Products

• [Journal Article] Expression of foreign aquaporin genes in lily pollen protoplasts (2011)
  • Author(s): Yamada,K., Yanada,K., Matsuzawa,A., Tanaka,I., Shiota,H.
  • Journal Title: Plant Biotechnol Volume: 28 Pages: 509-514
  • DOI: DOI:10.5511/plantbiotechnology.11.0922a
  • Peer Reviewed
• [Journal Article] Molecular morphological studies on pollen development using protoplasts (2011)
  • Author(s): Tanaka,I
  • Journal Title: Plant Morphol Volume: 23 Pages: 53-59
  • URL: http://square.umin.ac.jp/pl-morph/
  • Peer Reviewed
• [Journal Article] Characterization of bacterial-type phosphoenolpyruvate carboxylase expressed in male gametophyte of higher plants (2010)
  • Author(s): Igawa,T., Fujiwara,M., Tanaka,I.,Fukao,Y., Yanagawa,Y
  • Journal Title: BMC Plant Biology Volume: 10 Pages: 200-211
  • DOI: DOI:10.1186/1471-2229-10-200
  • Peer Reviewed
• [Journal Article] Nucleotide polymorphism in the carrot embryogenesis abundant geneDC8/ECP63 (2010)
  • Author(s): Shiota,H., Matsuzawa,A., Kurokawa,K., Sugimoto,G., Yonezawa,M., Tanaka,I.,kamada,H
  • Journal Title: Scientia Horticulturae Volume: 125 Pages: 767-770
  • DOI: DOI:10.1016/j.scienta.2010.05.001
  • Peer Reviewed
• [Journal Article] 花粉内栄養細胞の分化とクロマチン (2010)
  • Author(s): 田中一朗、最上則史、佐野弥生子
  • Journal Title: 横浜市立大学論叢自然科学系列 Volume: 60 Pages: 41-49
  • DOI: DOI:10.1042/CBI20090124.www.cellbiolint.org
• [Presentation] 朝倉さくら子、ニンジン液胞膜アクアポリン遺伝子 DcTIP3 の発現解析 (2012)
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-16
• [Presentation] テッポウユリ花粉に特異的なArgonaute(AGO)の発現解析 (2011)
  • Author(s): 新屋智尋
  • Organizer: 第29回日本植物細胞分子生物学会
  • Place of Presentation: 九州大学
  • Year and Date: 2011-09-07
• [Presentation] 細胞からみる花粉の使命 (2010)
  • Author(s): 田中一朗
  • Organizer: 本花粉学会第51回大会
  • Place of Presentation: 中央大学
  • Year and Date: 2010-10-09
• [Presentation] テッポウユリの花粉管伸長過程における栄養核クロマチンの変化 (2010)
  • Author(s): 佐野弥生子
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学
  • Year and Date: 2010-09-11
• [Presentation] テッポウユリ花粉におけるArgonaute 様遺伝子の単離と発現解析 (2010)
  • Author(s): 新屋智尋
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学
  • Year and Date: 2010-09-11
• [Presentation] プロトプラストを用いた花粉発生の分子形態学 (2010)
  • Author(s): 田中一朗
  • Organizer: 日本植物形態学会第22回大会
  • Place of Presentation: 中部大学
  • Year and Date: 2010-09-08
• [Book] 花粉の世界をのぞいてみたら (2012)
  • Author(s): 田中一朗
  • Total Pages: 335
  • Publisher: エヌ・ティー・エス
• [Book] 生物学辞典 (2010)
  • Author(s): 田中一朗
  • Total Pages: 1620
  • Publisher: 東京化学同人
• [Remarks]
  • URL: http://histone.sci.yokohama-cu.ac.jp/