Molecular cell biological studies on the evolution of male and female sex in algae.

2012 Fiscal Year Final Research Report

• Project/Area Number: 22570084
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biodiversity/Systematics
• Research Institution: University of Tsukuba
• Principal Investigator: MIYAMURA Shinichi 筑波大学, 生命環境系, 准教授 (00192766)
• Co-Investigator(Kenkyū-buntansha): KAWANO Shigeyuki 東京大学, 新領域創成科学研究, 教授 (70161338) ; MOTOMURA Taizo 北海道大学, 北方生物圏フィールド科学センター, 教授 (30183974)
• Project Period (FY): 2010 – 2012
• Keywords: 性 / 配偶子 / 雌雄性 / 藻類

Research Abstract

Spatial arrangement of gamete fusion site or mating structure was studied in ulvophycean green algae, Chaetomorpha spiralis, Monostroma angicava and chlorophycean green alga Gonium pectoral using electron microsco py. In all species, cell fusion site of the gamete was located on the same side of the eyespot in one sex type while it was located on the other side of the eyespot in the opposite mating type. These results support the proposal that sex specific arrangement of cell fusion site or mating structure may be a universal feature in chlorophyte algae. The relationship between the sex determination gene MID and sex specific arrangement of mating structure was studied using G. pectorale. In the MID+ gametes, mating structure was present on the same side as the eyespot while in the MID- gametes mating structure was present on the opposite side of the eyespot. These results are consistent with that of Chlamydmonas reinhardtii and suggest that the close relationship between presence or absence of MID and sex specific arrangement of mating structure in these algae.

Research Products

• [Journal Article] Localization and evolution of septins in algae. (2013)
  • Author(s): Yamazaki, T., Owari, S., Ohta, S., Sumiya, N., Yamamoto, M., Watanabe. K., Nagumo, T., Miyamura, S., and Kawano, S.
  • Journal Title: The Plant Journal Volume: 74 Pages: 605-614
  • DOI: DOI:10.1111/tpj.12147
  • Peer Reviewed
• [Journal Article] Sex specific sapatial arrangement of gamete fusion site in chlorophyte algae. (2012)
  • Author(s): Miyamura, S.
  • Journal Title: Aquabiology Volume: 34 Pages: 443-452
  • URL: http://aquabiology.blog93.fc2.com/category7-1.html
• [Journal Article] Ultrastructural study of plasmodesmata in the brown alga Dictyota dichotoma (Dictyotales, Phaeophyceae). (2012)
  • Author(s): Terauchi, M., Nagasato, C., Kajimura, N., Mineyuki, Y., Okuda, K., Kastaros, C. and Motomura, T
  • Journal Title: Planta Volume: 236 Pages: 1013-1026
  • DOI: DOI:10.1007/s00425-012-1656-4
  • Peer Reviewed
• [Journal Article] Ultrastructural analysis of flagellar development in plurilocular sporangia of Ectocarpus siliculosus (Phaeophyceaea) (2012)
  • Author(s): Fu, G., Nagasato, C., Ito, T., Muller, D.G., and Motomura, T.
  • Journal Title: Protoplasma Volume: 205 Pages: 261-272
  • DOI: DOI:10.1007/s00709-012-0405-7
  • Peer Reviewed
• [Journal Article] Three dimensional ultrastructural study of oil and astaxanthin accumulation during encystment in the green alga Haematococcus. (2012)
  • Author(s): Wayama, M., Ota, S., Matuura,H., Nango, N., Hirata, A., and Kawano, S.
  • Journal Title: PLOS One Volume: 8 Pages: e53618
  • URL: http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053618
  • Peer Reviewed
• [Journal Article] The role of multiple FtsZ rings in chloroplast division under oligotrophic and eutrophic conditions in the unicellular green alga, Nannochloris bacillaris (Chlorophyta, Trebouxiophyceae). (2012)
  • Author(s): Sumiya, N., Owari, S., Watanabe, K., and Kawano, S.
  • Journal Title: J. Phycol. Volume: 48 Pages: 1187-1196
  • DOI: DOI:10.1111/j.1529-8817.2012.01204.x
  • Peer Reviewed
• [Journal Article] Isolation of mitochondrial and plastid ftsZ genes and analysis 1 of the organelle targeting sequence in the diatom Chaetoceros neogracile (Diatoms,Bacillariophyceae) (2012)
  • Author(s): Nishikawa, T., Moriyama, Y., Sato, M., Sano, T.,Hasezawa, S., Ota, S., and Kawano, S.
  • Journal Title: Phycol. Res. Volume: 60 Pages: 123-136
  • DOI: DOI:10.1111/j.1440-1835.2012.00644.x
  • Peer Reviewed
• [Journal Article] Chloroplast division and differentially regulated expression of FtsZ1 and FtsZ2 in Nannochloris bacillalis (Chlorophyta, Trebouxiophyceae) (2012)
  • Author(s): Sumiya, N., Watanabe, K., Owari, S., Yamamot, M., and Kawano, S.
  • Journal Title: Cytologia Volume: 77 Pages: 59-66
  • URL: https://www.jstage.jst.go.jp/article/cytologia/77/1/77_59/_pdf
  • Peer Reviewed
• [Journal Article] Comparative immunofluorescence and ultrastructural analysis of microtubule organization in Uronema sp., Klebsormidium flaccidum (2012)
  • Author(s): Katsaros, C., Varvarigosa, V., Gachon, C. M. M., Jerry Brand, Motomura, T., Nagasato, C., and Kupper, F. C.
  • Journal Title: Protist Volume: 162 Pages: 315-331
  • DOI: Doi:org/10.1016/j.protis.2010.10.004
  • Peer Reviewed
• [Journal Article] DNA Content of Stichococcus bacillaris(Trebouxiophyceae, Chlorophyta) nuclei determined with laser scanning cytometry. (2011)
  • Author(s): Yamamoto, M., Handa, S. and Kawano, S.
  • Journal Title: Cytologia Volume: 76 Pages: 157-161
  • URL: https://www.jstage.jst.go.jp/article/cytologia/76/2/76_2_157/_pdf
  • Peer Reviewed
• [Journal Article] Isolation of chloroplast FtsZ and AtpC, and analysis of protein targeting into the complex chloroplast of the haptophyte Pavlova pinguis. (2010)
  • Author(s): Nishikawa, T., Kajitani, H., Sato, M., Mogi, Y., Moriyama, Y. and Kawano, S.Nishikawa, T., Kajitani, H., Sato, M., Mogi, Y., Moriyama, Y. and Kawano, S.
  • Journal Title: Cytologia Volume: 75 Pages: 203-210
  • URL: https://www.jstage.jst.go.jp/FF01S040Init?sourceurl=%2Farticle%2Fcytologia%2F75%2F2%2F75_2_203%2F_pdf
  • Peer Reviewed
• [Journal Article] Membrane fusion process and assembly of cell wall during cytokinesis in the brown alga, Silvetia babingtonii (Fucales, Phaeophyceae). (2010)
  • Author(s): Nagasato, C., Inoue, A., Mizuno, M., Kanazawa, K., Ojima, T., Okuda, K., and Motomura, T
  • Journal Title: Planta Volume: 232 Pages: 287-298
  • DOI: DOI:10.1007/s00425-010-1188-8
  • Peer Reviewed
• [Journal Article] Analysis of expressed sequence tags from the chrysophycean alga Ochromonas danica (Heterokontophyta) (2010)
  • Author(s): Terauchi, M., Kato, A., Nagasato, C. and Motomura, T.
  • Journal Title: Phycological Research Volume: 58 Pages: 217-221
  • DOI: DOI:10.1111/j.1440-1835.2010.00578.x
  • Peer Reviewed
• [Journal Article] Cytoplasmic inheritance in green algae: patterns, mechanisms and relation to sex type. (2010)
  • Author(s): Miyamura, S.
  • Journal Title: J. Plant Res. Volume: 123 Pages: 171-184
  • DOI: DOI:10.1007/s10265-010-0309-6
  • Peer Reviewed
• [Journal Article] Behavior of flagella and flagellar root systems in the planozygotes and settled zygotes of the green alga Bryopsis maxima Okamura (Ulvophyceae, Chlorophyta) with reference to spatial arrangement of eyespot and cell fusion site. (2010)
  • Author(s): Miyamura, S., Sakaushi, S., Horo, T. and Nagumo, T.
  • Journal Title: Phycol. Res. Volume: 58 Pages: 258-269
  • DOI: DOI: 10.1111/j.1440-1835.2010.00585.x
  • Peer Reviewed
• [Presentation] 緑藻Gonium pectorale の配偶子における接合装置の空間配置と交配型の関係 (2012)
  • Author(s): 梅澤 歩、横田真吾、宮村新一、南雲 保、河野重行、浜地貴志、野崎久義
  • Organizer: 日本藻類学会第36回大会
  • Place of Presentation: 札幌、北海道大学
  • Year and Date: 20120714-15
• [Presentation] イチョウ精子の構造と進化 (2011)
  • Author(s): 宮村新一
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川.旭川市神楽地区公共施設群
  • Year and Date: 20110921-23
• [Presentation] 緑藻Gonium pectorale の同形配偶子における細胞融合部位の性特異性 (2011)
  • Author(s): 横田真吾、宮村新一. 南雲 保
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京.東京大学
  • Year and Date: 20110917-19
• [Presentation] 海産緑藻オオハネモの接合における雌雄鞭毛装置と眼点の挙動 (2010)
  • Author(s): 宮村新一、南雲 保
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 春日井市、中部大学
  • Year and Date: 20100909-11
• [Presentation] 母細胞壁の界列と脱離の有無からみたトレボキシア藻の娘細胞連結 (2010)
  • Author(s): 山本真紀、半田信司、宮村新一、南 雲 保、河野重行
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 春日井市、中部大学
  • Year and Date: 20100909-11