Exploring evolution and figure of early fungal clade "LKM11"in oxygen-depleted environment

2012 Fiscal Year Final Research Report

• Project/Area Number: 22570105
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biodiversity/Systematics
• Research Institution: Higashichikushi Junior College
• Principal Investigator: NAGAHAMA Takahiko 東筑紫短期大学, 食物栄養学科, 講師 (10359169)
• Co-Investigator(Kenkyū-buntansha): NAGANO Yuriko 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 研究員 (30512917) ; MIYAZAKI Masayuki 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 技術研究副主事 (50399573)
• Project Period (FY): 2010 – 2012
• Keywords: 菌類 / LKM11 クレード / 初期進化

Research Abstract

For exploring early evolution of fungi, we investigated fungaldiversity in sediments colleted in eight lakes in west Japan. By using culture-independentmethod with fungal-specific PCR of SSU rDNA, we revealed an large extent of novel fungaldiversity in lacustrine environment. Four hundreds fungal phylotypes were obtained fromPCR clone libraries of six lakes successfully amplified. Novel (<97% identity) phylotypesare 213 (53.3%) in all fungal phylotypes. There were 187 phylotypes (46.8%) related toDikarya (Ascomycota + Basidiomycota). Whereas, Non-Dikarya fungi (Chytridiomycota +Blastocladiomycota + LKM11 etc) were 53.3%, 74.8% of which were estimated to be novelphylotypes with <97% affinities. All LKM11 phylotypes were novel. Early fungal lineagesoccupied a majority of all fungal phylotypes from the lacustrine environment in west Japanand indicated an unexpected diversity and novelty.

Research Products

• [Journal Article] Fungal diversity in deep-sea extreme environments (2012)
  • Author(s): Nagano, Y. & Nagahama, T.
  • Journal Title: Fungal Ecology Volume: 5巻 Pages: 463-471
  • DOI: DOI：10.1016/j.funeco.2012.01.004
  • Peer Reviewed
• [Journal Article] The Hadal Amphipod Hirondellea gigas Possessing a Unique Cellulase for Digesting Wooden Debris Buried in the Deepest Seafloor (2012)
  • Author(s): Kobayashi, H., Hatada, Y., Tsubouchi,T., Nagahama, T. & Takami, H.
  • Journal Title: PLoS ONE Volume: 7巻 Pages: 42727
  • DOI: DOI：10.1371/journal.pone.0042727
  • Peer Reviewed
• [Journal Article] Halosarpheia japonica sp. nov.(Halosphaeriales, Ascomycota) from marine habitats in Japan. (2012)
  • Author(s): Abdel-Wahab, M.A. & Nagahama, T.
  • Journal Title: Mycological Progress Volume: 11巻 Pages: 85-92
  • DOI: DOI；10.1007/s11557-010-0731-0
  • Peer Reviewed
• [Journal Article] Geofilum rubicundum gen. nov., sp. nov.,isolated from deep subseafloor sediment (2012)
  • Author(s): Miyazaki, M., Koide, O., Kobayashi, T.,Mori, K., Shimamura, S., Nunoura, T.,Imachi, H., Inagaki, F., Nagahama, T.,Nogi,Y.,Deguchi,S.& Takai,K.
  • Journal Title: International Journal of Systematic and Evolutionary Microbiology Volume: 62巻 Pages: 1075-1080
  • DOI: DOI：10.1099/ijs.0.032326-0
  • Peer Reviewed
• [Journal Article] Abdel‐Wahab, M. A. & Miyazaki, M.Molecular evidence that deep‐branching fungi are major fungal components in deep‐sea methane cold‐seep sediments (2011)
  • Author(s): Nagahama, T., Takahashi, E., Nagano,Y.
  • Journal Title: Environmental Microbiology Volume: 13巻 Pages: 2359-2370
  • DOI: DOI：10.1111/j.1462-2920.2011.02507.x
  • Peer Reviewed
• [Journal Article] Yeast extract stimulates production of glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma hubeiensis SY62 (2011)
  • Author(s): Konishi, M., Nagahama, T., Fukuoka, T.,Morita, T., Imura, T., Kitamoto, D.&Hatada, Y.
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 111巻 Pages: 702-705
  • DOI: DOI：10.1016/j.jbiosc.2011.02.004
  • Peer Reviewed
• [Journal Article] Microbulbifer chitinilyticus sp. nov. and Microbulbifer okinawensis sp. nov.,chitin-degrading bacteria isolated from mangrove forests (2011)
  • Author(s): Baba, A., Miyazaki, M., Nagahama, T.& Nogi, Y.
  • Journal Title: International Journal of Systematic and Evolutionary Microbiology Volume: 61巻 Pages: 2215-2220
  • DOI: DOI：10.1099/ijs.0.024158-0
  • Peer Reviewed
• [Journal Article] Gesasha (Halosphaeriales, Ascomycota), a new genus with three new species from the Gesashi mangroves in Japan (2011)
  • Author(s): Abdel-Wahab, M. A. & Nagahama, T.
  • Journal Title: Nova Hedwigia Volume: 92巻 Pages: 497-512
  • DOI: DOI：10.1127/0029-5035/2011/0092-0497
  • Peer Reviewed
• [Journal Article] 深海環境における真菌多様性 (2010)
  • Author(s): 長野由梨子&長濱統彦
  • Journal Title: 高圧力の科学と技術 Volume: 20巻 Pages: 321-329
  • URL: https://www.jstage.jst.go.jp/article/js hpreview/20/4/20_4_321/_pdf
  • Peer Reviewed
• [Journal Article] Fungal diversity in deep-sea sediments -the presence of novel fungal groups (2010)
  • Author(s): Nagano, Y., Nagahama, T., Hatada, Y.,Nunoura, T., Takami, H., Miyazaki, J.,Takai, K. & Horikoshi, K.
  • Journal Title: Fungal Ecology Volume: 3巻 Pages: 316-325
  • DOI: DOI：10.1016/j.funeco.2010.01.002
  • Peer Reviewed
• [Journal Article] Biosurfactant-producing yeast isolated from Calyptogena soyoae (deep-sea cold-seep clam) in the deep sea (2010)
  • Author(s): Konishi, M., Fukuoka, T., Nagahama, T.,Morita, T., Imura, T., Kitamoto, D. & Hatada, Y.
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 110巻 Pages: 169-175
  • DOI: DOI：10.1016/j.jbiosc.2010.01.018
  • Peer Reviewed
• [Book] Fungal Diversity in Deep-Sea Environments In Biology of Marine Fungi,Progress in Molecular and Subcellular Biology,Edited by C. Raghukumar (2012)
  • Author(s): Nagahama, T. & Nagano, Y. Cultured and Uncultured
  • Total Pages: 173-187
  • Publisher: Springer Berlin Heidelberg