2012 Fiscal Year Final Research Report
Regulation mechanism of nutrient assimilation and exocrine system which is achieved by the carbohydrate-recognition of pancreatic enzymes
| Project/Area Number |
22570111
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Ochanomizu University |
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Principal Investigator |
OGAWA Haruko お茶の水女子大学, 大学院・人間文化創成科学研究科, 教授 (90143700)
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| Co-Investigator(Renkei-kenkyūsha) |
KAWASAKI Nana 国立医薬品食品衛生研究所, 生物薬品部, 部長 (20186167)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 膵臓酵素 / 糖鎖認識 / 糖質消化 / 糖質吸収 / 外分泌調節 / 糖尿病 |
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| Research Abstract |
We found novel carbohydrate-binding activities of pancreatic enzymes; -amylase, trypsinogen/trypsin, and lipase, etc. The carbohydrate-binding sites of bovine trypsinogen and trypsin were identified by cocrystallization with Me -GalNAc, and X-ray crystallography. Expression, purification, and refolding methods of recombinant human pancreatic lipase were established for the first time using Escherichia coli. The interaction between -amylase and N-glycans in the BBM activated starch degradation to produce much more Glc on one hand, while suppressing a sharp increase in Glc absorption on the other. Therefore, the carbohydrate recognition of α-amylase was shown to playa key role in regulating Glc assimilation to maintain blood homeostasis in the intestine.
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[Journal Article] Functional regulation of sugar assimilation by N-glycan-specific interaction of pancreatic ・-amylase with glycoproteins of duodenal brush border membrane2012
Author(s)
Asanuma-Date, K., Hirano, Y., Le, N., Sano, K., Kawasaki, N., Hashii, N., Hiruta, Y., Nakayama, K., Umemura, M., Ishikawa, K., Sakagami, H., Ogawa, H
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Journal Title
J. Biol. Chem
Volume: 287
Pages: 23104-18
DOI
Peer Reviewed
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[Journal Article] Novel anti-HIV-1 activity produced by conjugating dextran with polyL-lysine2012
Author(s)
Nakamura, K., Ohtsuki, T., Hoshino, H., Tanamoto, K., Mori, H., Ushijima, H., Kawasaki, N., Akiyama, H., Yajima, T., Sakagami, H., Ogawa, H
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Journal Title
Antiviral Res
Volume: 94
Pages: 89-97
DOI
Peer Reviewed
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[Journal Article] Usefulness of specific antibodies to immobilize pyridylaminated N-glycans for solid-phase interaction analyses2011
Author(s)
Le, N., Kato, M., Kubo, H., Hirashima, Y., Sakagami, H., Ogawa H
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Journal Title
Nat Sci. Rept. Ochanomizu Univ. Tokyo
Volume: 61
Pages: 31-45
URL
Peer Reviewed
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[Journal Article] Survival of Hepatic Stellate Cell during Liver Regeneration is Regulated by the Changes in Glycosylation of Rat Vitronectin especially the Decreased Hypersialylation2010
Author(s)
Sano, K., Miyamoto, Y., Kawasaki, N., Hashii, N., Itoh, S., Yokoyama, M., Sato, C., Kitajima, K., Ogawa, H
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Journal Title
J. Biol. Chem
Volume: 285
Pages: 17301-17309
DOI
Peer Reviewed
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