Structure analysis of Nectin and Nectin like protein family

2012 Fiscal Year Final Research Report

• Project/Area Number: 22570115
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Osaka University
• Principal Investigator: SUZUKI Mamoru 大阪大学, たんぱく質研究所, 准教授 (40280507)
• Project Period (FY): 2010 – 2012
• Keywords: 構造生物 / 細胞接着分子

Research Abstract

We determined the crystal structures of the entire extracellular region of nectin-1,2,3. In the crystal, nectin formed a V-shaped homophilic dimer through the first Ig-like domain. Structure-based site-directed mutagenesis experimental results indicate that, in contrast with the previous notion, our structure represents a cis-dimer. Thus, our findings clearly reveal the structural basis for the cis-dimerization of nectins through the first Ig-like domains.

Research Products

• [Journal Article] Crystal structure of a Bombyx mori Sigma-1 class glutathione transferase exhibiting prostaglandin E synthase activity (2013)
  • Author(s): Yamamoto, K., Higashiura, A., Suzuki,M., Aritake, K.Urade, Y., Udome, N. Nakagawa, A
  • Journal Title: Biochim Biophys Acta Volume: 1830 Pages: 3711-3718
  • Peer Reviewed
• [Journal Article] Structural basis for catalytic activity of a silkworm Delta-class glutathione transferase (2012)
  • Author(s): Yamamoto, K., Usuda, K., Kakuta, Y., Makoto Kimura, M., Higashiura, A., Nakagawa, A., Suzuki, M
  • Journal Title: Biochim Biophys Acta. Volume: 1820 Pages: 1469-1474
  • Peer Reviewed
• [Journal Article] Beamline AR-NW12A: high-throughput beamline for macromolecular crystallography at the Photon Factory (2012)
  • Author(s): Chavas LM, Matsugaki N, Yamada Y, Hiraki M, Igarashi N, Suzuki M, Wakatsuki S
  • Journal Title: J Synchrotron Radiat Volume: 19 Pages: 450-454
  • Peer Reviewed
• [Journal Article] Crystal structure of the cis-dimer of nectin-1 : Implications for the architecture of cell-cell junctions (2011)
  • Author(s): Narita, H., Yamamoto, Y., Suzuki.M., Miyazaki, N., Yoshida, A., Kawai, K., Iwasaki, K., Nakagawa, A., Takai, Y., Sakisaka, T
  • Journal Title: The Journal of Biological Chemistry Volume: 286 Pages: 12659-12669
  • Peer Reviewed
• [Journal Article] Refolding, crystallization and preliminary X-ray crystallographic study of the whole extracellular regions of nectins (2011)
  • Author(s): Narita, H., Nakagawa, A., Yamamoto, Y., Sakisaka, T., Takai, Y., Suzuki.M.
  • Journal Title: Acta Crystallographica Section F Volume: 67 Pages: 344-348
  • Peer Reviewed
• [Presentation] ネクチン・ネクル分子群の構造解析 (2013)
  • Author(s): 成田宏隆・中川敦史・鈴木守
  • Organizer: 物構研サイエンスフェスタ
  • Place of Presentation: つくば
  • Year and Date: 20130314-15
• [Presentation] 細胞接着装置の制御に関わるLNX1PDZ2の構造・機能解析 (2012)
  • Author(s): 中倉 由香子, 秋吉 由佳里, 合田 名都子, 成田 宏隆, 鈴木 守, 天野 剛志, 浜田大三, 藤原 芳江, 中川 敦史, 古瀬 幹夫, 廣明 秀一
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 20121212-16
• [Presentation] Crystal Structure Analysis of Nectin and Nectin-Like-Molecule Family(II) (2012)
  • Author(s): Mamoru Suzuki, Hirotaka Narita, Atsushi Nakagawa
  • Organizer: Meeting of the American CrystallographicAssociation
  • Place of Presentation: Boston, MA, USA
  • Year and Date: 20120701-0728
• [Presentation] 細胞接着分子ネクチンおよびネクチン様分子のX線結晶構造 (2012)
  • Author(s): 成田宏隆・藤原芳江・中川敦史・鈴木 守
  • Organizer: 第29回PFシンポジウム
  • Place of Presentation: つくば
  • Year and Date: 20120315-16
• [Presentation] A little more until sunrise (2012)
  • Author(s): Mamoru Suzuki
  • Organizer: Sapporo Symposiumon Advanced Protein Crystallography.(招待講演)
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-17
• [Presentation] 細胞接着分子ネクチンおよびネクチン様分子のX線結晶構造解析 (2011)
  • Author(s): 成田 宏隆、中川 敦史、鈴木 守
  • Organizer: 日本結晶学会年会
  • Place of Presentation: 札幌
  • Year and Date: 20111124-25
• [Presentation] ネ クチン・ネクル分子群の構造解析の試み (2011)
  • Author(s): 成田 宏隆、中川 敦史、鈴木 守
  • Organizer: 第28回PFシンポジウム
  • Place of Presentation: つくば
  • Year and Date: 20110711-13
• [Presentation] Crystal structure analysis of nectin and nectin like molecule family (2011)
  • Author(s): Mamoru Suzuki, Hirotaka Narita, Atsushi Nakagawa
  • Organizer: Meeting of the American Crystallographic Association
  • Place of Presentation: ニューオリンズ,USA
  • Year and Date: 20110528-0602
• [Presentation] ネクチン・ネクル分子群の構造解析の試み (2011)
  • Author(s): 成田宏隆、中川敦史、鈴木守
  • Organizer: 第28回PFシンポジウム
  • Place of Presentation: つくば (震災のため中止、要旨はシンポジウムのWeb上に公開済み)
  • Year and Date: 20110314-15
• [Presentation] 細胞間接着・細胞運動に関わるタンパク質の構造解析に向けた取組み (2011)
  • Author(s): 成田宏隆、中川敦史、鈴木守
  • Organizer: 第24回日本放射光学会放射光科学合同シンポジウム
  • Place of Presentation: つくば
  • Year and Date: 20110107-10
• [Presentation] ネクチン3のC末端配列と結合したアファディンPDZドメインの構造解析 (2010)
  • Author(s): 藤原芳江、合田名都子、里村香織、成田宏隆、中川敦史、匂坂敏朗、鈴木守、廣明秀一
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
  • Place of Presentation: 神戸
  • Year and Date: 20101207-10
• [Presentation] 細胞間接着分子ネクチン-2の構造生物学的研究 (2010)
  • Author(s): 成田宏隆、中川敦史、岩崎憲治、匂坂敏朗、高井義美、鈴木守
  • Organizer: 日本結晶学会年会
  • Place of Presentation: 大阪
  • Year and Date: 20101203-05
• [Presentation] ネクチンファミリーの結晶構造解析 (2010)
  • Author(s): 成田宏隆、中川敦史、岩崎憲治、匂坂敏朗、高井義美、鈴木守
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌
  • Year and Date: 20100616-18
• [Remarks]
  • URL: http://www.protein.osaka-u.ac.jp/rcsfp/supracryst/suzuki/index_ja.html