2012 Fiscal Year Final Research Report
Structural analysis of supra-biomolecules by using ion mobility mass spectrometry
| Project/Area Number |
22570120
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Yokohama City University |
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Principal Investigator |
AKASHI Satoko 横浜市立大学, 大学院・生命ナノシステム科学研究科, 准教授 (10280728)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 質量分析 |
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| Research Abstract |
Structural study of supra-biomoleculeswere executed by using ion mobility mass spectrometry (IM-MS), which provides not only of masses but also of collision cross-sections of the supra-biomolecules. Since IM-MS is applicable to theprotein complexes that are difficult to crystallize, it was possible to analyze theirstability and conformational alteration by IM-MS. In the present study, a calculation method of collision cross sectionsof dummy residue models for noncrystallizable supra-biomolecules was established.In addition, it was proved by IM-MS and molecular dynamics (MD) simulation that behaviors of the folded and unfolded regions in the gas phase were remarkably varied.
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[Journal Article] Gas-phase structure of the histone multimers characterized by ion mobility mass spectrometry and molecular dynamics simulation2013
Author(s)
Saikusa, K., Fuchigami, S., Takahashi, K., Asano, Y., Nagadoi, A., Tachiwana, H., Kurumizaka, H., Ikeguchi, M., Nishimura, Y., *Akashi, S
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Journal Title
Anal. Chem
Volume: 85
Pages: 4165-4171
URL
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[Journal Article] Function of homo-and hetero-oligomers of human nucleoplasmin/nucleophosmin family proteins NPM1, NPM2, and NPM3 during sperm chromatin remodeling2012
Author(s)
*Okuwaki, M., Sumi, A., Hisaoka, M., Saotome-Nakamura, A., Akashi, S., Nishimura, Y., Nagata, K
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Journal Title
Nuleic Acid Res
Volume: 40
Pages: 4861-4878
DOI
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[Journal Article] Crystal Structures of Penicillin-Binding Protein 3 (PBP3) from Methicillin-Resistant Staphylococcus aureus in the Apo and Cefotaxime-Bound Forms.2012
Author(s)
Yoshida, H., Kawai, F., Obayashi, E., Akashi, S., Roper, D.I., Tame, J.R., *Park, S.Y
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Journal Title
J Mol Biol
Volume: 423
Pages: 351-364
DOI
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[Journal Article] Characterisation of an intrinsically disordered protein complex of Swi5-Sfr1 by ion mobility mass spectrometry and small-angle X-ray scattering2012
Author(s)
Saikusa, K., Kuwabara, N., Kokabu, Y., Inoue, Y., Sato, M., Iwasaki, H., Shimizu, T., Ikeguchi, M., *Akashi, S
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Journal Title
Analyst
Volume: 138
Pages: 1441-1449
DOI
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[Journal Article] Structural and biochemical analyses of the human PAD4 variant encoded by a functional haplotype gene2011
Author(s)
Horikoshi, N.,Tachiwana, H., Saito, K., Osakabe, A., Sato, M., Yamada, M., Akashi, S., Nishimura, Y., Kagawa, W., *Kurumizaka, H
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Journal Title
Acta Crystallogr D Biol Crystallogr
Volume: 67
Pages: 112-118
DOI
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[Journal Article] Fission yeast Swi5-Sfr1 complex, an activator of Rad51 recombinase, forms an extremely elongated Dogleg-shaped structure2011
Author(s)
Kokabu, Y., Murayama, Y., Kuwabara, N., Oroguchi, T., Hashimoto, H., Tsutsui, Y., Nozaki, N., Akashi, S., Unzai, S., Shimizu, T., Iwasaki, H., Sato, M., *Ikeguchi, M
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Journal Title
J. Biol. Chem
Volume: 286
Pages: 43569-43576
DOI
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[Journal Article] Crystal structure of human REV7 in complex with a human REV3 fragment and structural implication of the interaction between DNA polymerase・and REV12010
Author(s)
Hara, K., *Hashimoto H., Murakumo, Y.,Kobayashi, S., Kogame, T., Unzai, S., Akashi, S., Takeda, S., Shimizu, T., Sato, M
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Journal Title
J. Biol. Chem
Volume: 285
Pages: 12299-12307
DOI
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[Journal Article] Deimination stabilizes histone H2A/H2B dimers as revealed by electrospray ionization mass spectrometry2010
Author(s)
Shimoyama, S., Nagadoi, A., Tachiwana, H., Yamada, M., Sato, M., Kurumizaka, H., *Nishimura, Y., *Akashi, S
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Journal Title
J. Mass Spectrom
Volume: 45
Pages: 900-908
DOI
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[Journal Article] Guidelines for reporting the use of capillary electrophoresis in proteomics.2010
Author(s)
Domann, P.J., Akashi, S., Barbas, C., Huang, L., Lau, W., Legido-Quigley, C., McClean, S., Neususs, C., Perrett, D., Quaglia, M., Rapp, E., Smallshaw, L., Smith, N.W., Smyth, W.F., Taylor, C.F
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Journal Title
Nat. Biotechnol.
Volume: 28
Pages: 654-655
DOI
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[Presentation] What happens on the histone multimers in the gas phase?2012
Author(s)
Kazumi Saikusa, Sotaro Fuchigami, Kyohei Takahashi, Yuuki Asano, Aritaka Nagadoi, Hiroaki Tachiwana, Hitoshi Kurumizaka, Mitsunori Ikeguchi, Yoshifumi Nishimura, Satoko Akashi
Organizer
19th International Conference on Mass Spectrometry
Place of Presentation
Kyoto
Year and Date
20120716-31
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[Presentation] Characterization of histone multimers by ESI-MS2011
Author(s)
Shingo Shimoyama, Yuuki Asano, Kyohei Takahashi, Kazumi Saikusa, Aritaka Nagadoi, Hiroaki Tachiwana, Hitoshi Kurumizaka, Yoshifumi Nishimura, Satoko Akashi
Organizer
The 59th ASMS Conference on Mass Spectrometry and Allied Topics, poster
Place of Presentation
Denver
Year and Date
2011-06-02
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