2012 Fiscal Year Final Research Report
Elucidation of dynamic structural changes of protein antigens and antibodies in their interactions
| Project/Area Number |
22570121
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kyoto Prefectural University |
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Principal Investigator |
ODA Masayuki 京都府立大学, 大学院・生命環境科学研究科, 准教授 (20318231)
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| Co-Investigator(Renkei-kenkyūsha) |
UCHIYAMA Susumu 大阪大学, 大学院・工学研究科, 准教授 (90335381)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 分子認識 / 生体分子 / タンパク質 / 分析化学 / 免疫学 |
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| Research Abstract |
Antibodies recognize various types of antigens, different shapes and sizes, and are good targets for the analysis to elucidate the structural dynamics of proteins. In this study, I changed the structures of protein antigens by chemical modification and disulfide-bonddeletion, and analyzed how the antibodies could recognize the slight and dynamic differences in antigens using the methods of binding kinetics and thermodynamics. As a result, I could detect the plural states of protein antigens and antibodies in solution, and quantitatively determine the effects of protein fluctuations.
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[Journal Article] Evaluation of the conformational equilibrium of reduced hen egg lysozyme by antibodies to the native form.2010
Author(s)
Oda, M., Kitai, A., Murakami, A., Nishimura, M., Ohkuri, T., Abe, Y., Ueda, T., Nakamura, H., and Azuma, T.
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Journal Title
Arch. Biochem. Biophys.
Volume: 494
Pages: 145-150
Peer Reviewed
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