2012 Fiscal Year Final Research Report
Molecular basis of intracellular dynamics of type 2 myosin isoforms
| Project/Area Number |
22570129
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Keywords | ミオシン / 細胞運動 / 細胞骨格 / フィラメント / アイソフォーム |
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| Research Abstract |
Myosin II forms a folded conformation (10S form) in the inactivated state; however, the physiological significance of the 10S form is still unclear. To investigate the role of 10S form, we generated a mutant of myosin II, which cannot fold into a 10S form under physiological condition. The mutant myosin II was less dynamic by stabilizing the filamentous form, and accumulated in the posterior region of migrating cells. We proposed that the 10S form is necessary for maintaining inactive nonmuscle myosin II in an unassembled state and for recruitment of nonmuscle myosin II to a specific region of the cell.
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[Journal Article] Nonmuscle myosin II folds into a 10S form via two portions of tail for dynamic subcellular localization2013
Author(s)
Kiboku, T., Katoh, T ., Nakamura, A.,Kitamura, A., Kinjo, M., Murakami, Y ., and Takahashi, M
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Journal Title
Genes to Cells
Volume: 18巻(2号)
Pages: 90-109
DOI
Peer Reviewed
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[Journal Article] Enucleation of human erythroblasts involves nonmuscle myosin IIB2012
Author(s)
Ubukawa, K., Guo, Y-M., Takahashi, M., Hirokawa, M., Michishita, Y., Nara, M., Tagawa, H., Takahashi, N., Komatsuda, A., Nunomura, W., Takakuwa, Y., and Sawada, K
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Journal Title
Blood
Volume: 119巻(4号)
Pages: 1036-104
DOI
Peer Reviewed
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[Journal Article] Enhancement of myosin II/actin turnover at the contractile ringinduces slower furrowing in dividing HeLa cells2011
Author(s)
Kondo, T., Hamao, K., Kamijo, K., Kimura, H, Morita, M., Takahashi, M., and Hosoya, H
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Journal Title
Biochem. J
Volume: 435巻(3号)
Pages: 569-576
DOI
Peer Reviewed
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