Cell adhesion machineries on basement membrane laminins

2012 Fiscal Year Final Research Report

• Project/Area Number: 22570141
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Osaka University
• Principal Investigator: YAMADA Masashi 大阪大学, 蛋白質研究所, 助教 (90304055)
• Co-Investigator(Renkei-kenkyūsha): SEKIGUCHI Kiyotoshi 大阪大学, 蛋白質研究所, 教授 (50187845)
• Project Period (FY): 2010 – 2012
• Keywords: 細胞外マトリックス / 基底膜 / ラミニン / インテグリン / テトラスパニン / 細胞遊走 / 細胞極性 / 細胞内シグナル

Research Abstract

To elucidate the molecular mechanisms by which basement membrane laminins regulate various cellular functions such as growth, differentiation, survival and migration, we focused on cell adhesion complexes based on laminin -binding integrins. As a result of the proteomic analyses of the complexes, we found the possibility that tetraspanin-enriched structures are involved in the regulation of the rear-end retraction in migrating cells on laminins.

Research Products

• [Journal Article] Substrate-attached materials are enriched with tetraspanins and are analogous to the structures associated with rear-end retraction in migrating cells. (2013)
  • Author(s): Yamada, M., Mugnai, G., Serada, S., Yagi, Y., Naka, T., and Sekiguchi K
  • Journal Title: Cell Adh. Migr
  • Peer Reviewed
• [Journal Article] Nephronectin binds to heparan sulfate proteoglycans via its MAM domain (2013)
  • Author(s): Sato, Y., Shimono, C., Li, S., Nakano, I., Norioka, N., Sugiura, N., Kimata, K., Yamada, M., and Sekiguchi, K
  • Journal Title: Matrix Biol. Volume: 32 Pages: 188-195
  • Peer Reviewed
• [Journal Article] Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells (2012)
  • Author(s): Miyazaki, T., Futaki, S., Suemori, H., Taniguchi, T., Yamada, M., Kawasaki, M., Hayashi, M., Kumagai, H., Nakatsuji, N., Sekiguchi, K., and Kawase, E
  • Journal Title: Nat. Commun Volume: 3 Pages: 1236
  • Peer Reviewed
• [Journal Article] BIT/SHPS-1 promotes antiapoptotic effect of BDNF on low potassium-induced cell death of cultured cerebellar granule neurons (2011)
  • Author(s): Koshimizu, H., Suzuki, S., Araki, T., Yamada, M., Kojima, M., and Hatanaka, H.
  • Journal Title: Cell. Mol. Neurobiol Volume: 31 Pages: 1027-1032
  • Peer Reviewed
• [Presentation] ラミニン E8 フラグメントを用いたヒト ES/iPS 細胞の単一分散培養法 (2013)
  • Author(s): 宮崎隆道、二木杉子、末盛博文、谿口征雅、山田雅司、川崎美和、林麻利亜、熊谷英明、中辻憲夫、関口清俊、川瀬栄八郎
  • Organizer: 第12回日本再生医療学会
  • Place of Presentation: 横浜
  • Year and Date: 2013-03-23
• [Presentation] Recombinant human laminin E8 fragments (LM-E8s) support the efficient adhesion and expansion of dissociated human pluripotent stem cells under defined and xeno-free condition (2012)
  • Author(s): Miyazaki, T., Futaki, S., Suemori, H., Taniguchi, Y., Yamada, M., Kawasaki, M., Hayashi, M., Kumagai, H., Nakatsuji, N., Sekiguchi, K., and Kawase, E
  • Organizer: World Stem Cell Summit 2012
  • Place of Presentation: West Palm Beach, Florida, USA
  • Year and Date: 20121203-05
• [Presentation] Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells (2012)
  • Author(s): Miyazaki, T., Futaki, S., Suemori, H., Taniguchi, Y., Yamada, M., Kawasaki, M., Hayashi, M., Kumagai, H., Nakatsuji, N., Sekiguchi, K., and Kawase, E
  • Organizer: 10th Annual Meeting of International Society for Stem Cell Research
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20120613-16
• [Presentation] Reexamination of substrate-attached materials in relation to rear retraction of migrating cells (2012)
  • Author(s): 山田雅司、ムニャーニ・ガブリエル、世良田聡、仲哲治、関口清俊
  • Organizer: 第45回日本発生生物学会・第64回日本細胞生物学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2012-05-30
• [Presentation] ラミニン結合性インテグリンを基盤とする接着複合体構成蛋白質の解析 (2011)
  • Author(s): 亀村和昌、 山田雅司、八木芳子、 関口清俊
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] ムニャーニ・ガブリエル、世良田聡、仲哲治、 関口清俊 : Substrate-attached material において多く検出されるテトラスパニン CD151 の細胞遊走制御における役割 (2011)
  • Author(s): 山田雅司
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-24
• [Presentation] Proteomic analysis of substrate-attached materials, SAM,based on interaction of integrin ・3・1 with laminin-511 (2010)
  • Author(s): 山田雅司、Gabriele Mugnai、川村桂太、世良田聡、仲哲治、関口清俊
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪
  • Year and Date: 20100519-21
• [Presentation] ネフロネクチンは MAM ドメインを介してヘパラン硫酸プロテオグリカンと結合する (2010)
  • Author(s): 佐藤祐哉、 下野知性、 李紹良、 中野伊津子、乗岡尚子、大窪哲郎、林麻利亜、杉浦信夫、木全弘治、山田雅司、関口清俊
  • Organizer: 第33回日本分子生物学会年会、第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Remarks]
  • URL: http://www.protein.osaka-u.ac.jp/chemistry/