2012 Fiscal Year Final Research Report
Molecular mechanism of transmembrane electron transfer catalyzed by a plant cytochrome b561
| Project/Area Number |
22570142
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Kobe University |
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Principal Investigator |
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| Research Collaborator |
TAKEUCHI Fusako 神戸大学, 大学教育推進機構, 助教 (00448169)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 金属タンパク質 / アスコルビン酸 / シトクロム b561 / ヘムタンパク質 / 電子伝達反応 / ビタミン C |
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| Research Abstract |
We investigated Zea mayscytochrome b561 by employing the Pichia pastoris expression system to clarify the physiological roles of plant vacuole cytochromes b561 and the molecular mechanisms of their ascorbate-specific transmembrane electron transfer. We succeeded in expression and purification of Zea mayscytochrome b561. The detailed analyses of the electron transfer reactions with ascorbate and monodehydroascorbate radical by stopped-flow and pulse-radiolysis methods indicated that Zea mays cytochrome b561 has a similar molecular mechanism with that of animal neuroendocrine cytochrome b561 and that conserveｄ amino acid residues have important roles for such ascorbate-specific transmembrane electron transfer reactions.
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