2012 Fiscal Year Final Research Report
Analysis of the endocytic and intracellular trafficking pathway of cell surface receptors
| Project/Area Number |
22570145
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
TOSHIMA Jiro 東京理科大学, 基礎工学部生物工学科, 准教授 (00333831)
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| Project Period (FY) |
2010 – 2012
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| Keywords | エンドサイトーシス / 膜輸送 |
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| Research Abstract |
To elucidate the mechanisms how activated receptor is recruited to clathrin coated pits, we screened yeast genome library, and identified Bmh2p, yeast homologue of 14-3-3 protein, as a binding protein for yeast Ste2 GPCR. In addition, we tagged human CCR2B receptor with GFP and succeeded in expressing and analyzing endocytic pathway of the receptor in yeast cells. We also screened about 5000 gene deletion mutants and identified 200 of them that exhibited aberrant endocytic transport.
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