2012 Fiscal Year Final Research Report
Elucidation of the intracellular protein folding mechanism?In vivo protein interaction analysis?
| Project/Area Number |
22570146
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Kanagawa Institute of Technology |
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Principal Investigator |
KOIKE Ayumi 神奈川工科大学, 応用バイオ科学部, 教授 (20454176)
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| Project Period (FY) |
2010 – 2012
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| Keywords | タンパク質フォールディング / シャペロニン |
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| Research Abstract |
To elucidate the reaction mechanisms of GroEL, important in protein folding in the cell, I analyzed the GroEL / GroES interactions in cells using in vivo photo-crosslinking techniques. After cross-linking of the GroEL-GroES complexes formed in cells,they were analyzed by western blotting. As a result, “football” type reaction intermediate was detected. Furthermore, fluorescence correlation spectroscopy (FCS) using a GroES which fused fluorescent protein enabled to detect GroEL-GroES complexes in the cell.
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