2012 Fiscal Year Final Research Report
Structural and functional analysis of novel dyneins localized to the ciliary proximal region
| Project/Area Number |
22570157
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
YAGI Toshiki 東京大学, 大学院・医学系研究科, 講師 (40292833)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 鞭毛・繊毛 / ダイニン / 微小管 / ATP |
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| Research Abstract |
We analyzed the structure and function of minor-type dyneins whose axonemal contents are lower than other major-type dyneins. Here we found that one of three minor dyneins more strongly bind to microtubules than most of Chlamydomonas dyneins in the absence of ATP. In addition, using cryoelectron tomography and subtomogram averaging, we found that each minor dynein locates to the ciliary proximal region in place of a specific major dynein. Furthermore, we found that a novel gigantic dynein, which has been the last dynein heavy chain remained to be identified in Chlamydomonas, localizes to the proximal region of one of nine doublet microtubules. This is the first report that all the gene products of dynein heavy chain have been identified in the singleorganism.
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[Journal Article] A unified taxonomy for ciliary dyneins.2011
Author(s)
Hom EFY, Witman GB, Harris EH, Dutcher SK, Kamiya R., Mitchell DR, Pazour GJ, Porter ME, Sale WS, Wirchell M., Yagi T., King SM.
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Journal Title
Cytoskeleton
Volume: 68
Pages: 555-65
DOI
Peer Reviewed
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