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2012 Fiscal Year Final Research Report

Thermodynamics of protein denaturation at high temperatures more than 100℃

Research Project

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Project/Area Number 22570166
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

YUTANI Katsuhide  独立行政法人理化学研究所, タンパク質結晶構造解析研究グループ, 上級研究員 (90089889)

Co-Investigator(Renkei-kenkyūsha) MATSUURA Yoshinori  独立行政法人理化学研究所, タンパク質結晶構造解析研究グループ, リサーチアソシエイト (50513462)
Project Period (FY) 2010 – 2012
Keywords蛋白質変性の熱力学 / 蛋白質の安定性 / 蛋白質熱変性の可逆性 / 示差走査熱量計 / 超好熱菌由来蛋白質 / 疎水性相互作用 / イオン相互作用 / CutA1
Research Abstract

The heat-denaturation of proteins is usually irreversible at higher temperatures than around 70℃, because of aggregation after heat denaturation. Therefore, their thermodynamic characteristics are less well understood at the temperatures more than 70℃. In this study, we found that SH-free CutA1 mutant from E. coli and its hydrophobic mutants show very good reversibility of heat denaturation at pH 9. The denaturation temperatures of the hydrophobic mutants were 90-115℃. Then, we succeeded to experimentally obtain the thermodynamic parameters of protein denaturation over 100℃ by DSC.

  • Research Products

    (15 results)

All 2013 2012 2011 2010

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (3 results) Book (1 results)

  • [Journal Article] Effect of heavy atoms on the thermal stability of α-amylase from Aspergillus oryzae2013

    • Author(s)
      Michihiro Sugahara, et al., Katsuhide Yutani.
    • Journal Title

      PLOS One

      Volume: 8 Pages: e57432,1-7

    • Peer Reviewed
  • [Journal Article] Structure and function of a TetR family transcriptional regulator, SbtR, from Thermus thermophilus HB82013

    • Author(s)
      Yoshihiro Agari, et al., Katsuhide Yutani
    • Journal Title

      Proteins

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Role of charged residues in stabilization of Pyrococcus horikoshii CutA1, which has a denaturation temperature of nearly 150℃2012

    • Author(s)
      Yoshinori Matsuura, et al., Katsuhide Yutani
    • Journal Title

      FEBS Journal

      Volume: 279 Pages: 78-90

    • Peer Reviewed
  • [Journal Article] Structural Characterization of a Trapped Folding Intermediate of Pyrrolidone Carboxyl Peptidase from a Hyperthermophile2012

    • Author(s)
      Mineyuki Mizuguchi, et al., Katsuhide Yutani
    • Journal Title

      Biochemistry

      Volume: 51 Pages: 6089-6096

    • Peer Reviewed
  • [Journal Article] A Demetallation Method for IMP-1 Metallo-β-Lactamase with Restored Enzymatic Activity Upon Addition of Metal Ion(s)2011

    • Author(s)
      Yoshihiro Yamaguchi, et al., Katsuhide Yutani
    • Journal Title

      ChemBioChem

      Volume: 12 Pages: 1979-1983

    • Peer Reviewed
  • [Journal Article] Crystal structure of indole-3-glycerol phosphate synthase from Thermus thermophilus HB8: implications for thermal stability2011

    • Author(s)
      Bagautdin Bagautdinov and Katsuhide Yutani
    • Journal Title

      Acta Crystallographica Section D

      Volume: D67 Pages: 1054-1064

    • Peer Reviewed
  • [Journal Article] The catalytic efficiency (kcat/Km) of the class A β-lactamase Toho-1 correlates with the thermal stability of its catalytic intermediate analog2010

    • Author(s)
      Yasushi Nitanai, et al., Katsuhide Yutani
    • Journal Title

      Biochem. Biophys. Acta

      Volume: 1804 Pages: 684-691

    • Peer Reviewed
  • [Journal Article] Large conformational changes in Escherichia coli tryptophan synthaseβ_2 Subunit upon binding of PLP2010

    • Author(s)
      Kazuya Nishio, et al., Katsuhide Yutani
    • Journal Title

      FEBS J.

      Volume: 227 Pages: 2157-2170

    • Peer Reviewed
  • [Journal Article] Crystal structure of TTHA0061, an uncharacterized protein from Thermus thermophilus HB8, reveals a novel fold.2010

    • Author(s)
      Tomoyuki Tanaka, et al., Katsuhide Yutani
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 400 Pages: 258-264

    • Peer Reviewed
  • [Journal Article] Construction of robust bio-nanotubes using the controlled self-assembly of component proteins of bacteriophage T42010

    • Author(s)
      Norihiko Yokoi, et al., Katsuhide Yutani
    • Journal Title

      Small

      Volume: 6 Pages: 1873-1879

    • Peer Reviewed
  • [Journal Article] Remarkable improvement in the heat stability of CutA1 from Escherichia coli by rational protein design2010

    • Author(s)
      Yoshinori Matsuura, et al., Katsuhide Yutani
    • Journal Title

      J. Biochem.

      Volume: 148 Pages: 449-458

    • Peer Reviewed
  • [Presentation] Heat-stabilization strategy due to unusual amount of charged residues in CutA1 protein from hyperthermophile, which has a denaturation temperature of nearly 150℃2012

    • Author(s)
      Yoshinori Matsuura, et al., Katsuhide Yutani
    • Organizer
      9th International Conference on Protein Stabilization
    • Place of Presentation
      Lisbon, Portugal
    • Year and Date
      2012-05-03
  • [Presentation] Does hydrophobic interaction due to hydration contribute to protein stability even over 100℃.2011

    • Author(s)
      Yoshinori Matsuura, et al., Katsuhide Yutani
    • Organizer
      7th Asian Biophysics Association Symposium
    • Place of Presentation
      New Delhi, India
    • Year and Date
      2011-02-01
  • [Presentation] Calorimetry of heat-reversible CutA1 proteins with a denaturation temperature higher than 100℃2010

    • Author(s)
      Yoshinori Matsuura, et al., Katsuhide Yutani
    • Organizer
      21st IUPAC International Conference on Chemical Thermodynamics
    • Place of Presentation
      Tsukuba
    • Year and Date
      2010-08-02
  • [Book] 「熱量測定・熱分析ハンドブック第2版」 超好熱菌由来蛋白質の熱安定性2010

    • Author(s)
      油谷克英
    • Total Pages
      357
    • Publisher
      丸善KK

URL: 

Published: 2014-08-29  

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