2012 Fiscal Year Final Research Report
Analysis of the components of a novel coactivator.the NF complex, and its regulatory mechanisms
| Project/Area Number |
22570168
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
HISATAKE Koji 筑波大学, 医学医療系, 教授 (70271236)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUDA Aya 筑波大学, 医学医療系, 准教授 (50436276)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 転写 / コアクチベーター |
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| Research Abstract |
The transcriptional activities of the NF complexes (NF45-NF90 and NF45-NF110) are mediated by both the upstream enhancer and core promoter regions of the c-fos gene. Knockdown of the endogenous NF90/NF110 in mouse cells results in a diminished induction of c-fos transcription upon serum stimulation. Chromatin immunoprecipitation assays show that the NF complexes occupy the c-fos enhancer/promoter region before and after serum induction and that their occupancies within the coding region of the c-fos gene increase in parallel to that of RNAPII upon serum induction. Thus, the NF complexes appear to serve as multifunctional coactivators that coordinate different steps of gene expression to facilitate rapid response of inducible genes.
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