2012 Fiscal Year Final Research Report
Transcriptional regulation of HSF1 by RPA
| Project/Area Number |
22570171
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Keywords | HSF1 / RPA / FACT / がん |
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| Research Abstract |
Heat shock factor 1 (HSF1) plays roles in control and stressed conditions by gaining access to target elements, but mechanisms of HSF1 access are not well known in mammalian cells. I found the physical interaction between the wing motif of human HSF1 and replication protein A (RPA), which is involved in DNA metabolism. Depletion of RPA1 abolishes HSF1 access to the promoter of HSP70 in unstressed condition and delays its rapid activation in response to heat shock. The HSF1-RPA complex leads to preloading of RNA polymerase II and opens the chromatin structure by recruiting a histone chaperone, FACT. Furthermore, this interaction is required for melanoma cell proliferation.
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[Journal Article] Impaired hippocampal spinogenesis and neurogenesis and altered affective behavior in mice lacking heat shock factor 12011
Author(s)
Shusaku Uchida, Kumiko Hara, Ayumi Kobayashi, Mitsuaki Fujimoto, Koji Otsuki, Hirotake Yamagata, Teruyuki Hobara, Naoko Abe, Fumihiro Higuchi, Tomohiko Shibata, Shunsuke Hasegawa, Satoshi Kida, Akira Nakai, and Yoshifumi Watanabe
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Journal Title
Proc. Natl. Acad. Sci. U.S.A
Volume: 108
Pages: 1681-1686
Peer Reviewed
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