2012 Fiscal Year Final Research Report
Analysis of the ubiquitin-ligase complex in the endoplasmic reticulum membrane that regulates endoplasmic reticulum-associated degradation in mammals
| Project/Area Number |
22570183
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Kyoto University |
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Principal Investigator |
HOSOKAWA Nobuko 京都大学, 再生医科学研究所, 准教授 (00263153)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 小胞体 / 小胞体関連分解 / ユビキチンリガーゼ / タンパク質品質管理 / HRD1-SEL1L複合体 / レクチン |
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| Research Abstract |
Endoplasmic reticulum (ER) is an organelle in which v a r i o u s kinds of proteins are synthesized. Newly synthesized proteins that failed to obtain their native conformations are retrotranslocated out of the ER, and degraded by the cytoplasmic proteasome after the addition of u b i q u i t i n s b y t h e u b i q u i t i n l i g a s e c o m p l e x t h a t r e s i d e s in the ER membrane. In the present study, we have analyzed the stability of each component of the complex and found that the dynamic change of the component o f t h e c o m p l e x r e g u l a t e s the degradation of misfolded substrates.
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