2012 Fiscal Year Final Research Report
MembraneinducedalterationsofstructureandsignaltransductionmechanisminthePIP3-bindingdomains
| Project/Area Number |
22570191
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | University of Hyogo |
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Principal Investigator |
TUZI Satoru 兵庫県立大学, 大学院・生命理学研究科, 准教授 (60227387)
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| Co-Investigator(Kenkyū-buntansha) |
YAGISAWA Hitoshi 兵庫県立大学, 大学院・生命理学研究科, 准教授 (40192380)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 細胞内情報伝達 / 固体NMR 分光法 / PH ドメイン |
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| Research Abstract |
Conformations of the pleckstrin homology (PH) domain in solutionand at the membrane surface were examined by using solid-state NMR, circular dichroism(CD) and fluorescence spectroscopy. The PH domains form one of the largest domainfamilies known to regulate membrane association of host proteins. Drastic conformationalalterations including a transition of the secondary structure were detected for themembrane-bound PH domain of a signal transduction protein SWAP-70. Theseconformational alterations induced at the membrane surface are suggested to regulatefunctions of SWAP-70, such as intracellular relocalization in response to the cellularsignaling.
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