2012 Fiscal Year Final Research Report
Studies on the role of FiliGAP (RacGAP) in mesenchymal and amoeboid cell migration.
| Project/Area Number |
22570192
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Cell biology
|
|---|
| Research Institution | Kitasato University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SATO Koji 北里大学, 理学部, 助教 (70556901)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Keywords | 細胞運動 / 細胞接着 / 細胞極性 / 低分子量 GTP 結合タンパク質 / 細胞骨格 |
|---|
| Research Abstract |
FilGAP is a Rac-specific GAP that suppresses Rac-depedndent lamellipodia formation and cell spreading. We have investigated how FilGAP is involved in the control of mesenchymal and amoeboid migration of tumor cells in 2-dimensional (2D) and 3-dimensional (3D) environments. (1) We found that FilGAP may restrict the area of lamella formation at the front of migrating cells in 2D. (2) We found that FilGAP is necessary for Bleb formation and amoeboid movement in 3D. Expression of FilGAP stimulates invasion of MDA-MB-231 cells. (3) FilGAP is phosphorylated and activated by Rho/ROCK-signaling. We found that Phosphorylation of FilGAP induces Bleb formation of tumor cells in 3D environment.
|
