2012 Fiscal Year Final Research Report
Analysis of retinotectal projection and tectal layer formation
| Project/Area Number |
22570199
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Keywords | 網膜視蓋投射 / 視蓋層形成 / ニワトリ胚 / エレクトロポレーション / Engrailed |
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| Research Abstract |
In this study, we have shown that some part of the optic fibers run deep layers of the tectum from the first. These fibers are transient and disappear after birth. En2 is expressed in laminae g-j of SGFS in tecta of around E10. Misexpression of En2 showed that En2 expressing cells cannot stay in the superficial layers of the tectum. Time lapse analysis showed that En2 expressing cells went out of the superficial layer and settled in lamina i. The results indicate that En2 plays crucial roles in tectal layer formation. We found that layer IV, and V are composed of cells that have migrated tangentially, and that neuropilin1 is expressed in layer IV, V cells and sema3A is expressed in layer VI cells. Sema3A overexpression resulted in abnormal layer formation of the tectum. The results indicate that neuropilin-Semaphorin repulsive system plays crucial roles in tectal layer formation.
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[Journal Article] Coactosin accelerates cell dynamism by promoting actin polymerization2013
Author(s)
Hou, X., Katahira, T., Ohashi, K., Mizuno, K., Sugiyama, S., Nakamura, H.
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Journal Title
Dev Biol
Volume: 379
Pages: 53-63
DOI
Peer Reviewed
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[Journal Article] Otx2 is involved in the regional specification of the developing retinal pigment epithelium by preventing the expression of Sox2 and Fgf8, factors that induce neural retina differentiation2012
Author(s)
Nishihara, D., Yajima, I., Tabata, H., Nakai, M., Tsukiji, N., Katahira, T., Takeda, K., Shibahara, S., Nakamura, H., Yamamoto, H.
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Journal Title
DOI
Peer Reviewed
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[Journal Article] Conditional knockdown of target gene expression by tetracycline regulated transcription of double strand RNA2011
Author(s)
Hou, X., Omi, M., Harada, H., Ishii, S., Takahashi, Y., Nakamura, H.
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Journal Title
Dev Growth Differ
Volume: 53
Pages: 69-75
DOI
Peer Reviewed
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[Journal Article] Gene manipulation of chick embryos in vitro, EC culture, and long survival in transplanted eggs2010
Author(s)
Tanaka, J., Harada, H., Ito, K., Ogura, T., Nakamura, H.
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Journal Title
Dev Growth Differ
Volume: 52
Pages: 629-634
DOI
Peer Reviewed
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[Presentation] Conditional knockdown of target gene expression: Tet-on of transcription of double strand RNA. Chick 7: Avian Model Systems2012
Author(s)
Hou, X., Omi, M., Harada, H., Ishii, S., Takahashi, Y., Nakamura, H.
Organizer
7th International Chick Meeting
Place of Presentation
Nagoya
Year and Date
20121114-18
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