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2012 Fiscal Year Final Research Report

Integration of signaling pathways by Sbno1 in neurogenesis

Research Project

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Project/Area Number 22570202
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionTohoku University

Principal Investigator

KATSUYAMA Yu  東北大学, 大学院・医学系研究科, 講師 (10359862)

Co-Investigator(Kenkyū-buntansha) TERASHIMA Toshio  神戸大学, 大学院・医学研究科, 教授 (20101892)
Project Period (FY) 2010 – 2012
Keywords細胞分化
Research Abstract

Maintenance of the neural stem cells and neuronal differentiation are precisely regulated during brain development, and molecular mechanisms involved in this regulation have not been fully understood yet. We found that the homologue of Sbno1 is required for neuronal differentiation by gene-knockdown experiments in zebrafish. We next examined in vivo function of Sbno1 during mouse brain development. We examined expression pattern of Sbno1 protein using anti-Sbno1 antibodies. In the developing cerebral cortex, Sbno1 was observed in the nucleus of differentiating neurons near the marginal zone, and also in the cytoplasm of the cells in basal side including the ventricular zone. To achieve gene knockout specifically in the dorsal forebrain, we constructed Sbno1-floxed mice, and crossed these to Emx1Cre transgenic strain that expresses Cre-recombinase in the developing dorsal forebrain. Sbno1 conditional knockout (cKO) mice had a very thin cerebral cortex, when examined at postnatal day 10. Proliferation of the neural stem cells was slightly in the E12.5 Sbno1 cKO. We observed intermingling of the neural stem cells and differentiating neurons from E12 to E14 in Sbno1 cKO embryos, suggesting premature differentiation of the neurons. Cell death detected by TUNEL method and active caspase3 or p53 immunoreactivity was robustly occurred in cKO cortex. Cell death was observed in the Tuj1 positive differentiating neurons, but not in the Pax6 positive neural stem cells. These observations suggest that Sbno1 is essential for not only maintenance of the neural stem cells but also survival of differentiating neurons.

  • Research Products

    (32 results)

All 2013 2012 2011 2010

All Journal Article (9 results) (of which Peer Reviewed: 8 results) Presentation (22 results) Book (1 results)

  • [Journal Article] Dynamic changes in the gene expression of zebrafish Reelin receptors during embryogenesis and hatching period2012

    • Author(s)
      Imai, H., Oomiya, Y., Kikkawa, S., Shoji, W., Hibi, M., Terashima, T., Katsuyama, Y.
    • Journal Title

      Development, Growth & Differentiation

      Volume: 54(2) Pages: 253- 263

    • DOI

      doi:10.1111/j.1440-169X.2012.01327.x.

    • Peer Reviewed
  • [Journal Article] Subcortically and callosally projecting neurons are distinct neuronal pools in the motor corte of the reeler mouse2012

    • Author(s)
      Imai, H., Yamamoto, T., Katsuyama, Y., Kikkawa, S., Terashima, T.
    • Journal Title

      Kobe Journal of Medical Sciences

      Volume: 58(3) Pages: E86-95

    • URL

      http://www.med.kobe-u.ac.jp/journal/contents/58/E86.pdf.

    • Peer Reviewed
  • [Journal Article] Reelin欠損マウス大脳皮質細胞構築異常2012

    • Author(s)
      勝山裕
    • Journal Title

      東北医学誌

      Volume: 124 Pages: 82-84

    • URL

      http://www.igakukai.med.tohoku.ac.jp/journal/index.html

  • [Journal Article] Function of strawberry notch family genes in the zebrafish brain development2011

    • Author(s)
      Takano, A., Zochi, R., Hibi, M., Terashima, T., Katsuyama, Y.
    • Journal Title

      Kobe Journal of Medical Sciences

      Volume: 56(5) Pages: E220-230

    • URL

      http://www.med.kobe-u.ac.jp/journal/contents/56/E220.pdf.

    • Peer Reviewed
  • [Journal Article] RA-GEF-1 (rapgef2) is essential for proper development of the midline commissures2011

    • Author(s)
      Bilasy SE, Satoh T, Terashima T, Kataoka T.
    • Journal Title

      Neuroscience Research

      Volume: 71(3) Pages: 200-9

    • DOI

      doi:10.1016/j.neures.2011.08.004.

    • Peer Reviewed
  • [Journal Article] Dispersion of the neurons expressing layer specific markers in the reeler brain2010

    • Author(s)
      Dekimoto, H., Terashima, T., Katsuyama, Y.
    • Journal Title

      Development Growth & Differentiation

      Volume: 52 Pages: 181-193

    • DOI

      doi:10.1111/j.1440-169X.2009.01153.x.

    • Peer Reviewed
  • [Journal Article] Expression of strawberry notch family genes during zebrafish embryogenesis2010

    • Author(s)
      Takano, A., Zochi, R., Hibi, M., Terashima, T., Katsuyama, Y.
    • Journal Title

      Developmental Dynamics

      Volume: 239 Pages: 1789-1796

    • DOI

      doi:10.1002/dvdy.22287.

    • Peer Reviewed
  • [Journal Article] PKH26 is an excellent retrograde and anterograde fluorescent tracer characterized by a small injection site and strong fluorescence emission2010

    • Author(s)
      Kawaguchi, K., Katsuyama, Y., Kikkawa, S., Setsu, T., Terashima, T.
    • Journal Title

      Archives of Histology and Cytology

      Volume: 73(2) Pages: 65-72

    • URL

      https://www.jstage.jst.go.jp/articl e/aohc/73/2/73_2_65/_pdf.

    • Peer Reviewed
  • [Journal Article] Cortical layer V neurons in the auditory and visual cortices of normal, reeler and yotari mice2010

    • Author(s)
      Yoshihara, Y., Setsu, T., Katsuyama, Y., Kikkawa, S., Terashima, T., Maeda, K.
    • Journal Title

      Kobe Journal of Medical Sciences

      Volume: 56(2) Pages: E50-E59

    • URL

      http://www.med.kobe-u.ac.jp/journal/contents/56/E50.pdf.

    • Peer Reviewed
  • [Presentation] Prox1 expressing cells in the developing mouse dentate gyrus2013

    • Author(s)
      杉山拓、 勝山裕 、大隅典子
    • Organizer
      第6回神経発生討論会埼玉
    • Place of Presentation
      和光
    • Year and Date
      20130313-14
  • [Presentation] Sbno1 is involved in neuronal differentiation and survival of differentiating neurons in the cortical development2013

    • Author(s)
      勝山裕
    • Organizer
      第6回神経発生討論会
    • Place of Presentation
      和光
    • Year and Date
      20130313-14
  • [Presentation] Pa6 affects distribution of GABAernic interneurons in prefrontal cortex of mice2012

    • Author(s)
      中村龍司、羽場美弥、勝山裕、吉崎嘉一、大隅典子
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋
    • Year and Date
      2012-09-21
  • [Presentation] マウス大脳皮質の分子マーカーによる 細胞構築の観察Characterization of cytoarchitecture in the cerebral cortex of the mouse brain with molecular markers2012

    • Author(s)
      高橋揚子、宮崎美香、杉山拓、大隅典子、勝山裕
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋
    • Year and Date
      2012-09-21
  • [Presentation] Molecular features of dentate granule progenitors in the developing mouse hippocampus発生期マウス海馬歯状回における顆粒前駆細胞の発現分子解析2012

    • Author(s)
      杉山拓、 勝山裕 、大隅典子
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋
    • Year and Date
      2012-09-20
  • [Presentation] 哺乳類大脳皮質において Pax6 下流遺伝 子の Dmrta1 は Neurogenin2 を制御する。Dmrta1, a transcription factor downstream to Pax6 regulates Neurogenin2 expression in the mammalian cortex2012

    • Author(s)
      吉川貴子、高橋将文、 勝山裕 、大隅典子
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋
    • Year and Date
      2012-09-19
  • [Presentation] Sbno1 is required for neural stem cell morphogenesis and survival of differentiating neurons during cortical development2012

    • Author(s)
      Yu Katsuyama, Hideaki Imai, Taku Sugiyama, Noriko Osumi, Toshio Terashima
    • Organizer
      包括型脳科学研究推進支援ネットワーク夏のワークショップ
    • Place of Presentation
      仙台
    • Year and Date
      2012-07-26
  • [Presentation] Expression pattern of transcription factors of dentate granule progenitors in the developing mouse hippocampus2012

    • Author(s)
      Taku Sugiyama, Yu Katsuyama, NorikoOsumi
    • Organizer
      包括型脳科学研究推進支援ネットワーク 夏のワークショップ
    • Place of Presentation
      仙台
    • Year and Date
      2012-07-26
  • [Presentation] 胎生期由来介在ニューロンの発生に対 する転写制御因子 Pax6 の関与2012

    • Author(s)
      R. Nakamura, Y. Katsuyama, K. Yoshizaki, H. Haba, N. Osumi
    • Organizer
      包括型脳科学研究推進支援ネットワーク 夏のワークショップ
    • Place of Presentation
      仙台
    • Year and Date
      2012-07-26
  • [Presentation] Sbno1 is required for neural stemcell morphology and survival of the differentiating neurons during corticogenesis2012

    • Author(s)
      Hideaki Imai,Toshio Terashima, Yu Katsuyama
    • Organizer
      第5回神経発生討論会
    • Place of Presentation
      福井
    • Year and Date
      2012-03-15
  • [Presentation] 網羅的行動テストバッテリー~大脳皮質細胞構築の脳機能における 役割を例に~2012

    • Author(s)
      今井英明、 勝山 裕
    • Organizer
      脳科学若手の会 東北支部会
    • Place of Presentation
      仙台
    • Year and Date
      2012-02-20
  • [Presentation] 大脳皮質層構造の特異的な欠損は情動の低下を引き起こす2012

    • Author(s)
      今井英明、寺島俊雄、勝山裕
    • Organizer
      東北脳科学ウィンタースクール
    • Place of Presentation
      宮城
    • Year and Date
      2012-02-19
  • [Presentation] Sbno1 is required for growth of the cerebral cortex during embryogenesis2011

    • Author(s)
      Naoya Ryoyama, Hideaki Imai, ToshioTerashima, Yu Katsuyama
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      横浜
    • Year and Date
      2011-09-15
  • [Presentation] Essential function of Sbno1 in Notch signal suppression during cortical neuron differentiation2011

    • Author(s)
      Yu Katsuyama, Ai Takano, Naoya Ryoyama, Hideaki Imai, Noriko Osumi, Masahiko Hibi, Toshio Terashima
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      横浜
    • Year and Date
      2011-09-15
  • [Presentation] Negative regulation of Notch signal by Sbno1 is essential for neuronal differentiation2011

    • Author(s)
      Yu Katsuyama, Ai Takano, Taku Sugiyama, Hideaki Imai, MasahikoHibi, Toshio Terashima, Noriko Osumi
    • Organizer
      Neurogenesis (Abcam)
    • Place of Presentation
      神戸CDB理研
    • Year and Date
      2011-06-03
  • [Presentation] Notch signal inactivation by Sbno1 is essential for mouse neuronal differentiation2011

    • Author(s)
      Yu Katsuyama, Ai Takano, Naoya Ryoyama, Hideaki Imai, Noriko Osumi, Masahiko Hibi, Toshio Terashima
    • Organizer
      第44回日本発生生物学会
    • Place of Presentation
      沖縄、宜野湾
    • Year and Date
      2011-05-19
  • [Presentation] Negative regulation of Notch signal by Sbno1 is essential for neuronal differentiation ニューロン分化における Sbno1 による Notch シグナルの抑制2010

    • Author(s)
      勝山裕
    • Organizer
      BMB2010 (第33回日本分子生物学会大会)
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
  • [Presentation] Suppression of Notch signal by Sbno1 is essential for differentiation of mouse cortical neurons2010

    • Author(s)
      Ai Takano, Riyo Zochi, Naoya Ryoyama, Masahiko Hibi, Toshio Terashima, Yu Katsuyama
    • Organizer
      NEUROSCIENCE2010 (Society for Neuroscience)
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2010-11-15
  • [Presentation] Histological and behavioral analysis of telencephalic Dab1 deficient mice2010

    • Author(s)
      H. Imai, A. Takano, N. Ryoyama, K. Sakimura, K. Sugioka, T. Terashima, Y. Katsuyama
    • Organizer
      NEUROSCIENCE2010 (Society for Neuroscience)
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2010-11-15
  • [Presentation] 前脳特異的Dab1遺伝子機能欠損マウスを用いた組織学的・経行動学的解析2010

    • Author(s)
      今井英明,高野愛,猟山直也,崎村建司,杉岡幸三,寺島俊雄、勝山裕
    • Organizer
      NEURO2010(第33回日本神経科学会)
    • Place of Presentation
      神戸
    • Year and Date
      2010-09-02
  • [Presentation] Sbno1によるNotchの抑制はマウス大脳皮質ニューロン分化に必須である2010

    • Author(s)
      高野愛、蔵地理代、今井英明、猟山直也、日比正彦、寺島俊雄、勝山裕
    • Organizer
      NEURO2010 (第33回日本神経科学会)
    • Place of Presentation
      神戸
    • Year and Date
      2010-09-02
  • [Presentation] Sbno1 promotes differentiation of cortical neurons in mouse brain development2010

    • Author(s)
      Ai Takano, Toshio Terashima, Hideyuki Dekimoto, Masahiko Hibi, Yu Katsuyama.
    • Organizer
      第43回日本発生生物学会
    • Place of Presentation
      京都
    • Year and Date
      2010-06-21
  • [Book] Notchシグナリング、シグナル伝達キーワード事典、編集:山本雅、仙波憲太郎、山梨裕司2012

    • Author(s)
      勝山裕
    • Total Pages
      45-46
    • Publisher
      羊土社

URL: 

Published: 2014-08-29  

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