Studies on the localization of the signaling moleculeWnt and it's function in the cell

2012 Fiscal Year Final Research Report

• Project/Area Number: 22570206
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Kagoshima University
• Principal Investigator: SAKAI Masao 鹿児島大学, 大学院・理工学研究科, 教授 (40162268)
• Project Period (FY): 2010 – 2012
• Keywords: アフリカツメガエル / Wnt / 小胞体 / lineage tracer / KDEL / chordin / シグナリング

Research Abstract

The aim of the present study is to show that Xwnt8, which has been shown to substitute the dorsal determinant in Xenopus early development, can act within the cell which inherit it. This study was carried out using morphological, immunohistochemical, lineage tracing and in situ hybridization techniques. We have shown, for the first time, that the signal transduction can occur within the cell, in a cell autonomous manner.

Research Products

• [Journal Article] Analysis of Head-Defects Caused by Hypergravity in Early Xenopus Embryos (2013)
  • Author(s): Makoto Yanagisawa, Keiko Kashiwagi, Hideki Hanada, Tadashi Shinkai, Satoshi Yoshitome, Hideo Kubo, Masao Sakai, Hirotada Fujii, Masamichi YamashitaAkihiko Kashiwagi, Nobuaki Furuno, Minoru Watanabe
  • Journal Title: Biological Sciences in Space Volume: 26 Pages: 1-6
  • Peer Reviewed
• [Journal Article] High cell-autonomy of the anterior endomesoderm viewed in blastomere fate shift during regulative development in the isolated right halves of four-cell stage Xenopus embryos (2012)
  • Author(s): Masaaki Koga, Takuro Nakashima, Shintaro Matsuo, Ryu Takeya, Hideki Sumimoto, Masao Sakai, Hiroshi Kageura
  • Journal Title: Development Growth and Differentiation Volume: 7 Pages: 717-729
  • Peer Reviewed
• [Presentation] Is chordin expressed only in the cells that received Xwnt8 mRNA (2012)
  • Author(s): Masao Sakai, Eriko Motomura, Tomohiro Narita, Shin-ichiro Nishimatsu, Tsutomu Nohno
  • Organizer: 14th International Xenopus Conference
  • Place of Presentation: フランス
  • Year and Date: 2012-09-12
• [Presentation] Isolated ectoderm is fated to die in early Xenopus embryos (2011)
  • Author(s): Hirotaka Kato, Ayumi Horai, Eriko Motomura, Masao Sakai
  • Organizer: 44th Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: 沖縄
  • Year and Date: 2011-05-20
• [Presentation] Expression of chordin in inverted embryos of Xenopus (2011)
  • Author(s): Eriko Motomura, Tomohiro Narita, Shin-ichiro Nishimatsu, Susumu Nohno Masao Sakai
  • Organizer: 44th Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: 沖縄
  • Year and Date: 2011-05-20
• [Presentation] Lonesome death of ectoderm (2010)
  • Author(s): Masao Sakai, Ayumi Horai, Hirotaka Kato, Yusuke Higashi, Eriko Motomura
  • Organizer: 13th International Xenopus Conference
  • Place of Presentation: カナダ
  • Year and Date: 2010-09-14
• [Presentation] Dorsalization by Wnt signaling occurs cell-autonomously in early Xenopus embryo (2010)
  • Author(s): Eriko Motomura, Narita Tomohiro, Shin-ichiro Nishimatsu, Tsutomu Nohno, Masao Sakai
  • Organizer: 13th International Xenopus Conference
  • Place of Presentation: カナダ
  • Year and Date: 2010-09-14
• [Remarks]
  • URL: http://www.sci.kagoshima-u.ac.jp/~garu/