2012 Fiscal Year Final Research Report
Functional analysis of grimp, a gene involved in early regeneration of Enchytraeus japonensis (Enchytraeidae, Oligochaete)
| Project/Area Number |
22570208
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Nihon University |
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Principal Investigator |
NORO Chikako 日本大学, 生産工学部, 教授 (80311356)
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| Co-Investigator(Renkei-kenkyūsha) |
TOCHINAI Shin 北海道大学, 理学研究, 教授 (20111148)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 幹細胞 / 再生 / 遺伝子 / タンパク質 / ヤマトヒメミミズ |
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| Research Abstract |
grimp gene plays an important role in early stage of regeneration in Enchytraeus japonensis. grimp mRNA is detected transiently from 3 to 12 h post amputation only in mesodermal stem cells called neoblasts and mesodermal lineage cells. In order to clarify the function and cellular localization of grimp protein, we took approaches for the production, purification and characterization of recombinant protein. We also studied the physical role of grimp by introducing grimp-GFP fusion gene into mammalian stem cells. The results suggested that the half-life of the grimp-GFP was relatively short, there could be a mechanism for selective degradation of grimp protein.
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