Evolution of retinoic acid-dependent developmental regulatory system in chordates

2012 Fiscal Year Final Research Report

• Project/Area Number: 22570214
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Evolutionary biology
• Research Institution: Kochi University
• Principal Investigator: FUJIWARA Shigeki 高知大学, 教育研究部自然科学系, 教授 (40229068)
• Co-Investigator(Renkei-kenkyūsha): SASAKURA Yasunori 筑波大学, 生命環境科学研究科, 准教授 (10400649)
• Project Period (FY): 2010 – 2012
• Keywords: レチノイン酸 / Hox1 / 進化 / 遺伝子発現制御 / 脊索動物

Research Abstract

We demonstrated that retinoic acid activates expression of the Hox1 gene in ascidian embryos. We also showed that retinoic acid and Hox1 contributed to organogenesis of ascidian larvae. The larvacean Oikopleura dioica does not have retinoic acid synthase and retinoic acid receptor in their genome, even though it is a close relative of ascidians. We found in the present study that both larvaceans and ascidians have retinoic acid-independent mechanism to activate transcription of Hox1. We hypothesized that the common ancestor of larvaceans and ascidians had both retinoic acid-dependent and independent pathways for the Hox1 activation. Larvaceans are thought to have survived the loss of retinoic acid-related genes, probably because they had two redundant pathways.

Research Products

• [Journal Article] Identification of a retinoic acid-responsive neural enhancer in the Ciona intestinalis Hox1 gene (2013)
  • Author(s): Kanda, M., Ikeda, T. & Fujiwara, S
  • Journal Title: Dev. Growth Differ Volume: Vol. 55 Pages: 260-269
  • DOI: doi: 10.1111/dgd.12033
  • Peer Reviewed
• [Journal Article] Retinoic acid-driven Hox1 is required in the epidermis for forming the otic/atrial placodes during ascidian metamorphosis (2012)
  • Author(s): Sasakura, Y., Kanda, M., Ikeda, T., Horie, T., Kawai, N., Ogura, Y., Yoshida, R., Hozumi, A., Satoh, N. & Fujiwara, S
  • Journal Title: Development Volume: Vol. 139 Pages: 2156-2160
  • DOI: doi: 10.1242/dev.080234
  • Peer Reviewed
• [Journal Article] RACK1 regulates mesenchymal cell recruitment during sexual and asexual reproduction of budding tunicates (2012)
  • Author(s): Tatzuke, Y., Sunanaga, T., Fujiwara, S.& Kawamura, K.
  • Journal Title: Dev. Biol Volume: Vol. 368 Pages: 393-403
  • DOI: doi: 10.1016/j.ydbio.2012.06.006
  • Peer Reviewed
• [Journal Article] Expression and function of myc during asexual reproduction of the budding ascidian Polyandrocarpa misakiensis (2011)
  • Author(s): Fujiwara, S., Isozaki, T., Mori, K. & Kawamura, K
  • Journal Title: Dev. Growth Differ Volume: Vol. 53 Pages: 1004-1014
  • DOI: doi: 10.1111/j.1440-169X.2011.01312.x
  • Peer Reviewed
• [Journal Article] The ANISEED database: digital representation, formalization and elucidation of a chordate developmental program (2010)
  • Author(s): Tassy, O., Dauga, D., Daian, F., Sobral, D., Robin, F., Khoueiry, P., Salgado, D., Fox, V., Caillol, D., Schiappa, R., Laporte, B., Rios, A., Luxardi, G., Kusakabe, T., Joly, J. S., Darras, S., Christiaen, L., Contensin, M., Auger, H., Lamy, C., Hudson, C
  • Journal Title: Genome Res Volume: Vol. 20 Pages: 1459-1468
  • DOI: doi: 10.1101/gr.108175.110
  • Peer Reviewed
• [Journal Article] Identification of chondroitin/dermatan sulfotransferases in the protochordate, Ciona intestinalis (2010)
  • Author(s): Tetsukawa, A., Nakamura, J. & Fujiwara, S.
  • Journal Title: Comp. Biochem. Physiol Volume: Part B Vol. 157 Pages: 205-212
  • DOI: doi: 10.1016/j.cbpb.2010.06.009
  • Peer Reviewed
• [Journal Article] Regeneration of the gut requires retinoic acid in the budding ascidian Polyandrocarpa misakiensis (2010)
  • Author(s): Kaneko, N., Katsuyama, Y., Kawamura, K. & Fujiwara, S.
  • Journal Title: Dev. Growth Differ Volume: Vol. 52 Pages: 457-468
  • DOI: doi: 10.1111/j.1440-169X.2010.01184.x
  • Peer Reviewed
• [Presentation] Transcriptional regulatory mechanism of the Hox1 gene in Ciona intestinalis and Oikopleura dioica (2012)
  • Author(s): Kanda, M., Canestro, C., Ikeda, T. &Fujiwara, S
  • Organizer: Asia-Pacific Developmental Biology Conference
  • Place of Presentation: 台北市, 台湾
  • Year and Date: 2012-10-06
• [Presentation] カタユウレイボヤ胚におけるグリコサミノグリカン硫酸転移酵素の機能解析 (2012)
  • Author(s): 中村惇・藤原滋樹
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-15
• [Presentation] Transcriptional regulatory mechanism of Hox1 gene in urochordates (2012)
  • Author(s): Kanda, M., Canestro, C. & Fujiwara, S
  • Organizer: BSCB/BSDB/JSDB Joint Spring Meeting
  • Place of Presentation: Coventry市,イギリス
  • Year and Date: 2012-04-17
• [Presentation] Devising a gene silencing method in Ciona intestinalis (2011)
  • Author(s): Nishioka, M. & Fujiwara, S
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] ミサキマメイタボヤの無性生殖におけるmyc遺伝子の発現と機能 (2011)
  • Author(s): 礒崎崇臣・森京子・川村和夫・藤原滋樹
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-22
• [Presentation] カタユウレイボヤ胚の神経管形成におけるcdx遺伝子の役割 (2010)
  • Author(s): 川島大典・三田薫・藤原滋樹
  • Organizer: 日本分子生物学会第33回年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] 脊索動物ホヤの表皮においてレチノイン酸 → Hox1カスケードはotic placode相同器官の形成に必須である (2010)
  • Author(s): 笹倉靖徳・神田美幸・池田拓・堀江健生・河合成道・小椋陽介・吉田麗子・保住暁子・佐藤矩行・藤原滋樹
  • Organizer: 日本分子生物学会第33回年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] カタユウレイボヤ胚における動物半球特異的遺伝子のエンハンサー解析 (2010)
  • Author(s): 堀川陽輔・石田聡美・藤原滋樹
  • Organizer: 日本分子生物学会第33回年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] ホヤの発生学 (2010)
  • Author(s): 藤原滋樹・笹倉靖徳・神田美幸
  • Organizer: フォーラム2012/衛生薬学・環境トキシコロジー
  • Place of Presentation: 名古屋・招待講演
  • Year and Date: 2010-10-26