ANALYSIS OF EVOLUTIONARY MECHANISM OF DLX3 GENE CO-OPTION IN DEVELOPMENT OF NOVEL APPENDAGES OF VERTEBRATES

2012 Fiscal Year Final Research Report

• Project/Area Number: 22570218
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Evolutionary biology
• Research Institution: National Institute of Genetics
• Principal Investigator: SUMIYAMA Kenta 国立遺伝学研究所, 集団遺伝研究系, 助教 (00370114)
• Project Period (FY): 2010 – 2012
• Keywords: 遺伝子進化 / シスエレメント進化

Research Abstract

Through Dlx3-4 gene enhancer analysis, we found that (1) the core element of brachial arch enhancer I37-2 was conserved between mice and platypus, and branchial arch activity was acquired in placental mammal lineage. (2) I37-1 that shows limbs and hair follicles enhancer activities is quite ancient, conserved even in amphioxus. The core motif plays an important role in Dlx3 gene co-option in development of limbs and hair follicles

Research Products

• [Journal Article] "Theria-specific homeodomain and cis-regulatory element evolution of the Dlx3-4 bigene cluster in 12 different mammalian species." (2013)
  • Author(s): Kenta Sumiyama,Tsutomu Miyake,Jane Grimwood,Andrew Stuart,Mark Dickson,Jeremy Schmutz, Frank H.Ruddle,Richard M.Myers,and Chri T. Amemiya
  • Journal Title: Journal of Experimental Zoology Part B: Molecular and Developmental Evolution Volume: 318 Pages: 369-350
  • DOI: 10.1002/jez.b.22469
  • Peer Reviewed
• [Journal Article] "The Medaka zic1/zic4 Mutant Provides Molecular Insights into Teleost Caudal Fin Evolution." (2012)
  • Author(s): Yuuta Moriyama, Toru Kawanishi Ryohei Nakamura Tatsuya Tsukahara, Kenta Sumiyama, Maximiliano L. Suster, Koichi Kawakami, Atsushi Toyoda, Asao Fujiyama, Yuuri Yasuoka, Yusuke Nagao, Etsuko Sawatari, Atsushi Shimizu, Yuko Wakamatsu Masahiko Hibi, Masanori Taira, Masataka Okabe, Kiyoshi Naruse, Hisashi Hashimoto, Atsuko Shimada, and Hiroyuki Takeda.
  • Journal Title: Current Biology Volume: 22(7) Pages: 601-607
  • Peer Reviewed
• [Journal Article] "A living fossil in the genome of a living fossil: Harbinger transposons in the coelacanth genome" (2012)
  • Author(s): Jeramiah J. Smith, Kenta Sumiyama and Chris T. Amemiya
  • Journal Title: Molecular Biology and Evolution Volume: 29(3) Pages: 985-993
  • DOI: 10.1093/molbev/msr267
  • Peer Reviewed
• [Journal Article] "Loss-of-function mutation in a repressor module of human- specifically activated enhancer HACNS1." (2011)
  • Author(s): Kenta Sumiyama and Naruya Saitou
  • Journal Title: Molecular Biology and Evolution Volume: 28(11) Pages: 3005-3007
  • DOI: 10.1093/molbev/msr231
  • Peer Reviewed
• [Presentation] "Evolution of Cis-regulatory Elements in the Vertebrate Dlx Bigene Cluster System" (2011)
  • Author(s): Kenta Sumiyama
  • Organizer: Symposium of Genomic Conservation and Diversity of Organisms ~Beyond the NGS time of Life Science~
  • Place of Presentation: Tainan,Taiwan.
  • Year and Date: 2011-12-10
• [Presentation] "Theria-specific evolution in both coding and noncoding region of the Dlx4 gene." (2011)
  • Author(s): Kenta Sumiyama
  • Organizer: Annual Meeting for the Society for Molecular Biology and Evolution (SMBE 2011)
  • Place of Presentation: 京都
  • Year and Date: 2011-07-27
• [Presentation] "Functional evaluation of cis-regulatory elements and their evolution in the vertebrate Dlx3-7 bigene cluster." (2010)
  • Author(s): Kenta Sumiyama
  • Organizer: NAIST Global COE International Symposium 2010: Plasticity in Development and Evolution
  • Place of Presentation: 奈良
  • Year and Date: 2010-11-11
• [Book] <series モデル動物利用マニュアル> 生物機能モデルと新しいリソース・ リサーチツール 第6節 新しい生殖工学技術第4項 トランスポゾンを使った 新しい高効率トランスジェニックマウス作製法 (2011)
  • Author(s): 隅山健太、川上浩一
  • Publisher: エル・アイ・シー
• [Remarks] 研究者ホームページ(研究内容紹介)
  • URL: http://sayer.lab.nig.ac.jp/~sumiyama/