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2012 Fiscal Year Final Research Report

Comparative genetical analysis among South American indigenous populations

Research Project

  • PDF
Project/Area Number 22570231
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied anthropology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

DANJO Inaho  独立行政法人理化学研究所, 細胞材料開発室, 協力研究員 (30415228)

Project Period (FY) 2010 – 2012
Keywords南米 / 先住民族 / 少数民族 / B-LCL / B細胞株 / ゲノム解析 / マイクロサテライト / アレイCGH
Research Abstract

We established B lymphoblastoid cell lines (EBV-B cell) from peripheral blood mononuclear cell stocks obtained from 550 South American indigenous individuals. We performed analysis of genetical relationships among tribes to make clear the migration rout of mongoloid populations from Eurasia to South America. Before starting genetical analysis, we checked the genomic stability of EBV-B cells during establishment and long-term culture periods, and showed that the cells retain stable genome structures by genetical studies. For the genetical analysis, samples were divided into 5 groups according to the geographical location, and then figured out the genetical features with forensic microsatellite markers. The results showed that each group retained smaller heterogeneity and specific allele frequency patterns for each microsatellite locus. These results suggest that Amerind populations are not so homogenous as a whole but can be divided into some genetically-diverse groups.

  • Research Products

    (14 results)

All 2013 2012 2011 2010

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (7 results) Book (4 results)

  • [Journal Article] Dominant expansion of a cryptic subclone possessing an abnormal karyotype occurs in B lymphoblastoid cell lines during culture2013

    • Author(s)
      I.Danjoh, R.Shirota, T.Hiroyama, Y. Nakamura
    • Journal Title

      Cytogenetics and Genome Research

      Volume: 139 Pages: 88-96

    • Peer Reviewed
  • [Journal Article] Development of a robust method for establishing B cell lines using Epstein-Barr Virus2012

    • Author(s)
      I.Danjoh, H.Sone, R.Shirota, T. Hiroyama, Y.Nakamura
    • Journal Title

      In Vitro Cellular and Developmental Biology - Animal

      Volume: 48 Pages: 393-402

    • Peer Reviewed
  • [Journal Article] The Sonoda-Tajima Cell Collection, a human genetics research resource with emphasis on South American indigenous populations2011

    • Author(s)
      I.Danjoh, K.Saijo, T.Hiroyama, Y. Nakamura
    • Journal Title

      Genome Biology and Evolution

      Volume: 3 Pages: 272-283

    • Peer Reviewed
  • [Presentation] Epstein-Barr virusを用いたB細胞株樹立の効率に影響を与える因子の探索2012

    • Author(s)
      檀上稲穂、城田涼子、中村幸夫
    • Organizer
      日本分子生物学会
    • Place of Presentation
      福岡市
    • Year and Date
      20121211-14
  • [Presentation] Development of a robust method for establishing B cell lines using Epstein-Barr Virus2012

    • Author(s)
      I.Danjoh, R.Shirota Y.Nakamura
    • Organizer
      The American Society of Human Genetics Annual Meeting, San Francisco
    • Place of Presentation
      USA
    • Year and Date
      20121106-11
  • [Presentation] Development of a robust method for establishing B cell lines using Epstein-Barr Virus2012

    • Author(s)
      I.Danjoh, Y.Nakamura
    • Organizer
      日本癌学会
    • Place of Presentation
      札幌市
    • Year and Date
      20120919-21
  • [Presentation] Genomic stability of B lymphoblastoid cell lines established from the Sonoda-Tajima Cell Collection, the collection of South American indigenous populations2011

    • Author(s)
      I.Danjoh, K.Saijo, M.Nagayoshi, Y. Nakamura
    • Organizer
      International Congress of Human Genetics
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      20111011-15
  • [Presentation] 南米モンゴロイド系先住民族コレクションより樹立したB細胞株のゲノム安定性2010

    • Author(s)
      檀上稲穂、西條薫、永吉満利子、中村幸夫
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸市
    • Year and Date
      20101207-10
  • [Presentation] Collection of peripheral blood mononuclear cells obtained from Amerindians and other Mongoloid minority groups2010

    • Author(s)
      I.Danjoh, K.Saijo, K.Fukami-Kobayashi, Y.Nakamura
    • Organizer
      The American Society of Human Genetics Annual Meeting
    • Place of Presentation
      Washington D.C. USA
    • Year and Date
      20101102-06
  • [Presentation] 南米モンゴロイド系先住民族コレクションより樹立したB細胞株のゲノム安定性2010

    • Author(s)
      檀上稲穂、西條薫、永吉満利子、中村幸夫
    • Organizer
      日本人類学会
    • Place of Presentation
      北海道伊達市
    • Year and Date
      20101001-1003
  • [Book] がん細胞株・不死化細胞を用いた研究, 目的別で選べる細胞培養プロトコール、中村幸夫編2012

    • Author(s)
      檀上稲穂
    • Total Pages
      40-45
    • Publisher
      羊土社
  • [Book] ゲノムインキュベーターとしての細胞を用いた研究, 目的別で選べる細胞培養プロトコール、中村幸夫編2012

    • Author(s)
      檀上稲穂
    • Total Pages
      46-51
    • Publisher
      羊土社
  • [Book] Bリンパ芽球様細胞株(B-LCL)-樹立培養方法および維持培養方法, 目的別で選べる細胞培養プロトコール、中村幸夫編2012

    • Author(s)
      檀上稲穂
    • Total Pages
      176-186
    • Publisher
      羊土社
  • [Book] Clinical application of molecular diagnosis in cancer, radiation effect, and human diseases2010

    • Author(s)
      K.Hiyama, E.Hiyama, I.Danjoh
    • Total Pages
      21-34
    • Publisher
      Public databases useful for molecular diagnosis. Edited by Eiso Hiyama and Keiko Hiyama

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Published: 2014-08-29  

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