2012 Fiscal Year Final Research Report
Comparative genetical analysis among South American indigenous populations
| Project/Area Number |
22570231
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied anthropology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
DANJO Inaho 独立行政法人理化学研究所, 細胞材料開発室, 協力研究員 (30415228)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 南米 / 先住民族 / 少数民族 / B-LCL / B細胞株 / ゲノム解析 / マイクロサテライト / アレイCGH |
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| Research Abstract |
We established B lymphoblastoid cell lines (EBV-B cell) from peripheral blood mononuclear cell stocks obtained from 550 South American indigenous individuals. We performed analysis of genetical relationships among tribes to make clear the migration rout of mongoloid populations from Eurasia to South America. Before starting genetical analysis, we checked the genomic stability of EBV-B cells during establishment and long-term culture periods, and showed that the cells retain stable genome structures by genetical studies. For the genetical analysis, samples were divided into 5 groups according to the geographical location, and then figured out the genetical features with forensic microsatellite markers. The results showed that each group retained smaller heterogeneity and specific allele frequency patterns for each microsatellite locus. These results suggest that Amerind populations are not so homogenous as a whole but can be divided into some genetically-diverse groups.
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Research Products
(14 results)
