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2012 Fiscal Year Final Research Report

Evolutional divergence and functional non-coding RNA analysis of transposon-like gene

Research Project

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Project/Area Number 22580005
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionTottori University

Principal Investigator

TOMITA Motonori  鳥取大学, 農学部, 准教授 (70207611)

Project Period (FY) 2010 – 2012
Keywordsムギ類 / トランスポゾン / 進化 / RNA / 構造変異 / 翻訳領域 / 染色体 / クラスター
Research Abstract

Evolutional mobilization of the transposon Revolver was analyzed by using wild-type barley adapted on the warm-dry and cool-wet slopes of “Evolution Canyon” in Israel. A 0.7-kb RNA molecule was highly transcribed in the wild-type barleyon the warm-dry slope, whereas various RNA molecules of 1-2 kb were transcribed at low levels in the barley on the cold-wet slope, indicating drastic variation due to the ecological environment and recent movable activity of Revolver. Noncoding RNA variants of Revolver with, for example, a long or short first exon and a deletion of the second intron were isolated. The transcription initiation site, the protein synthesized in E. coli, and the chromosomal gene cluster of Revolver were clarified.

  • Research Products

    (8 results)

All 2013 2012 2011

All Journal Article (2 results) Presentation (4 results) Book (2 results)

  • [Journal Article] Rye chromosome- specific polymerase chain reaction products developed by primers designed from the EcoO109I recognition site2012

    • Author(s)
      Tomita, M., and Seno, A.
    • Journal Title

      Genome

      Volume: 55(5) Pages: 370-382

  • [Journal Article] Genomic, RNA, and ecological divergences of the Revolvertransposon- like multi-gene family in Triticeae2011

    • Author(s)
      Tomita, M., Okutani, A., Beiles, A., and Nevo, E.
    • Journal Title

      BMC Evolutionary Biology

      Volume: 11 Pages: 269

    • DOI

      DOI:10.1186/1471-2148-11-269

  • [Presentation] Divergent PCR products of Revolver transposon showing cluster-like chromosomal distribution2013

    • Author(s)
      Tomita, M., and Tanaka, E
    • Organizer
      日本育種学会第123回講演会, 育種学研究15
    • Place of Presentation
      東京農業大学
    • Year and Date
      20130300
  • [Presentation] Cluster-like chromosomal distribution of Revolver transposon and its transcription initiation site in Secale cereale2012

    • Author(s)
      Tomita, M., and Kanzaki, T
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡
    • Year and Date
      20121200
  • [Presentation] Revolverトランスポゾン様多重遺伝子族のゲノム、RNAおよび生態における多様な変異, 育種学研究142012

    • Author(s)
      富田因則、Nevo, E
    • Organizer
      日本育種学会第120回講演会
    • Place of Presentation
      宇都宮大学
    • Year and Date
      20120300
  • [Presentation] Sequence-tagged rye chromosome-specific polymerase chain reaction products developed by primers designed from the Eco0109I recognition site2011

    • Author(s)
      Tomita, M., and Seno, A.
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      20111200
  • [Book] 植物のゲノム育種.新バイオの扉 "未来に拓く生物工学"2013

    • Author(s)
      富田因則, 高木正道・池田友久
    • Publisher
      裳華房
  • [Book] Recent patent on Revolver-2: a novel transposon-like gene useful for chromosome tags of rye. In New Developments in Chromatin Research2012

    • Author(s)
      Tomita, M, Eds. Neil M. Simpson and Valerie J. Stewart
    • Publisher
      Nova Science Publishers

URL: 

Published: 2014-08-29  

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