2012 Fiscal Year Final Research Report
Metabolic engineering of fatty acidcomposition in soybean by using TILLING
| Project/Area Number |
22580006
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Saga University |
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Principal Investigator |
ANAI Toyoaki 佐賀大学, 農学部, 准教授 (70261774)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 突然変異 / 逆遺伝学 |
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| Research Abstract |
TILLING is one of the most attractive methods for screening novel mutant lines with reverse genetics approach in recent years.Inthis study aimed at improving fatty acid composition of soybean oil by metabolic engineering, I have tried to isolate novel mutant lines and to combine their mutant alleles. As a result, I have isolated several mutant lines corresponding to acyl-ACP thioesterase, stearoyl-ACP desaturase and microsomal omega-3 fatty acid desaturase genes, and successfully developed novel fatty acid mutant lines with more than 80% oleic acid or less than 1.5% linolenic acid in seed oil.
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[Journal Article] 2013 年 Genetic Variation in Soybean at the Maturity Locus E4 Is Involved in Adaptation to Long Days at High Latitudes Agronomy2013
Author(s)
Y. Tsubokura, H. Matsumura, M. Xu, B. Liu, H. Nakashima, T. Anai, F. Kong, X. Yuan, H. Kanamori, Y. Katayose, R. Takahashi, K. Harada and J. Abe
Volume
3
Pages
117-134
DOI
Peer Reviewed
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[Journal Article] 2012 年 Positional cloning and characterization reveal the molecular basis for soybean maturity locus E1, which regulates photoperiodic flowering2012
Author(s)
Z. Xia, S. Watanabe, T. Yamada, Y. Tsubokura, H. Nakashima, H. Zhai, T. Anai, S. Sato, T. Yamazaki, S. Lu, H. Wu, S. Tabata and K. Harada
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Journal Title
Proceedings of the National Academy of Sciences of the United States of America
Volume: 109
Pages: E2155-E2164
DOI
Peer Reviewed
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[Journal Article] 2011 年 A map-based cloning strategy employing a residual heterozygous line reveals that the GIGANTEA gene is involved in soybean maturity and flowering2011
Author(s)
S. Watanabe, Z. Xia, R. Hideshima, Y. Tsubokura, S. Sato, N. Yamanaka, R. Takahashi, T. Anai, S. Tabata, K. Kitamura, K. Harada
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Journal Title
Genetics
Volume: 188
Pages: 395-407
DOI
Peer Reviewed
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