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2011 Fiscal Year Final Research Report

Characterization of MYB genes regulating anthocyanin biosynthesis in Asiatic hybrid lily

Research Project

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Project/Area Number 22580022
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticulture/Landscape architecture
Research InstitutionHokkaido University

Principal Investigator

YAMAGISHI Masumi  北海道大学, 大学院・理学研究院, 准教授 (40210348)

Project Period (FY) 2010 – 2011
Keywordsスカシユリ / 花色 / 遺伝子発現制御 / 品種間差異 / 形質発現
Research Abstract

A single dominant locus determines anthocyanin biosynthesis in the tepals of Asiatic hybrid lilies(Lilium spp.); however the gene that determines this trait has not been previously reported. Furthermore, anthocyanin colour hue in tepals varies among cultivars, but the mechanisms underlying this variation are not known. Here, we show that LhMYB12, which is homologous to petunia An2, determines anthocyanin pigmentation in tepals and that the level of LhMYB12 transcription affects the quantity of anthocyanin pigments, producing colour hue variation in tepals. The pink-tepal cultivars 'Montreux' and 'Renoir' were heterozygous for the LhMYB12 gene. Their F_1 population segregated 3 : 1 for the presence or absence of anthocyanin in tepals, and LhMYB12 cosegregated perfectly with the presence of anthocyanin, indicating that LhMYB12 determines anthocyanin biosynthesis in tepals. Among 7 cultivars, a single anthocyanin cyanidin 3-0-0-rutinoside was detected in tepals displaying light pink, pink-red, dark red, or chocolate brown hues. However, concentrations differed according to the following order of colour hue : light pink<pink-red<dark red<chocolate brown, suggesting that colour hue variation in tepals is strongly related to anthocyanin quantities. The accumulation of LhMYB12 transcripts in tepals varied among 7 cultivars in the same order, and transcription levels were positively correlated with pigment quantities. This suggests that the levels of transcription of LhMYB12 vary among the different cultivars and subsequently affect the quantities of pigment in tepals. These results indicate that LhMYB12 determines anthocyanin pigmentation and generates the variation in anthocyanin-colour hue in lily tepals.

  • Research Products

    (12 results)

All 2012 2011 2010 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (6 results)

  • [Journal Article] Oriental hybrid lily 'Sorbonne' homologue of LhMYB12 regulates anthocyanin biosyntheses in flower tepals and tepal spots2011

    • Author(s)
      Masumi Yamagishi
    • Journal Title

      Molecular Breeding

      Volume: 28 Pages: 381-389

    • DOI

      DOI:10.1007/s11032-010-9490-5

    • Peer Reviewed
  • [Journal Article] Elevated temperature inhibits anthocyanin biosynthesis in the tepals of an Oriental hybrid lily via the suppression of LhMYB12 transcription2011

    • Author(s)
      Yun-Song Lai, Masumi Yamagishi and Takashi Suzuki
    • Journal Title

      Scientia Horticulturae

      Volume: 132 Pages: 59-65

    • DOI

      DOI:10.1016/j.scienta.2011.09.030

    • Peer Reviewed
  • [Journal Article] Two R2R3-MYB genes, homologues of petunia AN2, regulate anthocyanin biosyntheses in flower tepals, tepal spots and leaves of Asiatic hybrid lily2010

    • Author(s)
      Masumi Yamagishi, Yoshihiro Shimoyamada, Takashi Nakatsuka and Kiyoshi Masuda
    • Journal Title

      Plant & Cell Physiology

      Volume: 51 Pages: 463-474

    • DOI

      doi:10.1093/pcp/pcq011

    • Peer Reviewed
  • [Journal Article] Carotenoid composition and changes in expression of carotenoid biosynthetic genes in tepals of Asiatic hybrid lily2010

    • Author(s)
      Masumi Yamagishi, Sanae Kishimoto and Masayoshi Nakayama
    • Journal Title

      Plant Breeding

      Volume: 129 Pages: 100-107

    • DOI

      DOI:10.1111/j.1439-0523.2009.01656.x

    • Peer Reviewed
  • [Journal Article] Pigment accumulation and transcription of LhMYB12 and anthocyanin biosynthesis genes during flower development in the Asiatic hybrid lily(Lilium spp.)

    • Author(s)
      Yun-Song Lai, Yoshihiro Shimoyamada, Masayoshi Nakayama and Masumi Yamagishi
    • Journal Title

      Plant Science

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] The transcription factor LhMYB12 determines anthocyanin pigmentation in the tepals of Asiatic hybrid lilies(Lilium spp.) and regulates pigment quantity

    • Author(s)
      Masumi Yamagishi, Yusuke Yoshida and Masayoshi Nakayama
    • Journal Title

      Molecular Breeding

      Volume: (in press)

    • DOI

      DOI:10.1007/s11032-011-9675-6

    • Peer Reviewed
  • [Presentation] Pigment accumulation and transcription of LhMYB12 and anthocyanin biosynthesis genes during flower development of Asiatic hybrid lily(Lilium spp.)2012

    • Author(s)
      Yun-Song Lai, Yoshihiro Shimoyamada, Masayoshi Nakayama and Masumi Yamagishi
    • Year and Date
      20120328-29
  • [Presentation] Transcriptions of MYB Gene and Anthocyanin Biosynthesis Genes In Oriental Hybrid Lily 'Marrero' Are Suppressed by High Temperature2011

    • Author(s)
      Yun-Song Lai and Masumi Yamagishi
    • Organizer
      ASHS Annual Conference
    • Place of Presentation
      Waikoloa Village, Waikoloa, Hawaii
    • Year and Date
      20110925-28
  • [Presentation] ユリ(Lilium)花被片に認められる2種類の斑点について2011

    • Author(s)
      山崖璽登・赤木航一
    • Year and Date
      20110924-25
  • [Presentation] スカシユリ品種の'モントルー'と'ルノアール'の交雑に由来するF1集団におけるアントシアニン色素蓄積と肋MB12遺伝子の分離2011

    • Author(s)
      山岸真澄・吉田祐輔・中山真義
    • Year and Date
      20110320-21
  • [Presentation] オリエンタルハイブリッドユリの肋M}四12ホモローグは花被片におけるアントシアニン生合成を制御している2010

    • Author(s)
      山岸真澄
    • Organizer
      日本育種学会第118回講演会
    • Year and Date
      20100924-25
  • [Presentation] オリエンタルハイブリッドユリ花被片においてアントシアニン生合成を制御しているR2R3-M遺伝子2010

    • Author(s)
      昭遺伝子。山岸真澄
    • Organizer
      園芸学会平成22年度秋期大会研究発表
    • Year and Date
      20100919-20

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Published: 2013-07-31  

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