2012 Fiscal Year Final Research Report
Immunoelectron microscopy using aldehyde fixation with oxygen- bubbling for highly preserving protein in pathogen-infected anduninfected plant tissues
| Project/Area Number |
22580048
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant pathology
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| Research Institution | Kobe University |
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Principal Investigator |
朴 杓允 神戸大学, 大学院・農学研究科, 名誉教授 (20147094)
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| Co-Investigator(Kenkyū-buntansha) |
IKEDA Kenichi 神戸大学, 大学院・農学研究科, 准教授 (40437504)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 酸素バブリング / 感染応答タンパク / 糖タンパク / グルタルアルデヒド / 微細構造 / 冠根 / トルイジン青 / 液胞 |
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| Research Abstract |
A new chemical fixation method was developed successfully in this project. On fixing plants with glutaraldehyde together with oxygen bubbling, the higher preservation of glycoproteins was achieved, resulting in observing fine ultrastructure under electron microscope. The cell images involving membranes and matrix of organelles, cytoplasm and cell components were excellent. However, it was not yet apparent whether the bubbling fixation was good enough to the preservation of protein for immunoelectron microscopy or not.
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[Journal Article] Zinc compounds potentiated mycovirus transmission by attenuation of heterogenic incompatibility in Rosellinia necatrix.2013
Author(s)
Ikeda, K., Inoue, K., Kida,C., Uwamori,T., Sasaki, A., Kanematsu, S., Park, P
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Journal Title
Applied and Environmental Microbiology
Volume: (in press)
Peer Reviewed
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[Journal Article] Dysfunction of mitotic cell division at shoot apices triggered severe growth abortion in interspecific hybrids between tetraploid wheat and Aegilops tauschii2012
Author(s)
Hatano,H., Mizuno N.,Matsuda,R.,Shitsukawa, N.,Park, P., Takumi, S.
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Journal Title
New Phytologist
Volume: 194
Pages: 1143-1154
Peer Reviewed
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[Journal Article] In situlocalization of avenanthramide A and its biosynthetic enzyme in oat leaves infected with the crown rust fungus2011
Author(s)
Uchihashi,K,. Nakayashiki,H., Okamura,K., Ishihara, A., Tosa,Y. Park,P., Mayama, S.
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Journal Title
Puccinia coronataf . sp.Avenae, Physiological and Molecular Plant Pathology
Volume: 76
Pages: 173-181
Peer Reviewed
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[Journal Article] Genetic analysis of barrage line formation during mycelial incompati- bility in Rosellinia necatrix2011
Author(s)
Ikeda,K., Inoue, K., Nakamura, H.,Hamanaka, T., Ohta,T., Kitazawa,H.,Kida,C.,Kanematsu, S., Park, P
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Journal Title
Fungal iology
Volume: 15
Pages: 80-86
Peer Reviewed
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