2012 Fiscal Year Final Research Report
Study on geographical distribution and adaptation mechanisms for environmental factors of soybean-nodulating bradyrhizobia
| Project/Area Number |
22580068
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | University of Miyazaki |
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Principal Investigator |
SAEKI Yuichi 宮崎大学, 農学部, 教授 (50295200)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 土壌生物 / 分子生態 |
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| Research Abstract |
We investigated the relationship between the genetic diversity of indigenous soybean-nodulating bradyrhizobia and their geographical distribution in the United States using nine soil isolates from eight states. The bradyrhizobia were inoculated on three soybean Rj genotypes (non-Rj, Rj2Rj3, and Rj4). We analyzed their genetic diversity and community structure by means of restriction-fragment length polymorphisms of PCR amplicon to target the 16S-23S rRNA gene internal transcribed spacer region, using 11 USDA Bradyrhizobium strains as reference strains. We also performed diversity analysis, multi-dimensional scaling analysis based on the Bray-Curtis index, and polar ordination analysis to describe the structure and geographical distribution of the soybean-nodulating bradyrhizobial community. The major clusters were Bradyrhizobium japonicum Bj123, in the northern U.S., and Bradyrhizobium elkanii in the middle to southern regions. Dominance of bradyrhizobia in a community was generally larger for the cluster belonging to B. elkanii than for the cluster belonging to B. japonicum. The indigenous American soybean-nodulating bradyrhizobial community structure was strongly correlated with latitude. Our results suggest that this community varies geographically.
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Research Products
(12 results)
